Isolation of nucleic acid
First Claim
1. A method of isolating genomic DNA from a sample containing intact cells, said method comprising(a) contacting said sample containing intact cells with a detergent and magnetic particles, said magnetic particles comprising an organic polymer, and whereby soluble genomic DNA from said sample is bound to the surface of said particles in a sequence-independent manner in the presence of the detergent and absence of any chaotropic agent, and(b) separating said magnetic particles with bound genomic DNA from the sample.
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Abstract
The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a detergent and a solid support, whereby soluble nucleic acid in said sample is bound to the support, and separating said support with bound nucleic acid from the sample. Where the method of the invention is used to isolate DNA, it may conveniently be couple with a further step to isolate RNA from the same sample.
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Citations
24 Claims
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1. A method of isolating genomic DNA from a sample containing intact cells, said method comprising
(a) contacting said sample containing intact cells with a detergent and magnetic particles, said magnetic particles comprising an organic polymer, and whereby soluble genomic DNA from said sample is bound to the surface of said particles in a sequence-independent manner in the presence of the detergent and absence of any chaotropic agent, and (b) separating said magnetic particles with bound genomic DNA from the sample.
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20. A method of isolating genomic DNA from cells in a sample, said method comprising (a) obtaining cells from said sample by immunomagnetic separation;
- (b) producing a lysate by contacting said cells with a detergent and magnetic particles in the absence of any chaotropic agent, the magnetic particles comprising an organic polymer whereby soluble genomic DNA in said lysate is bound to the surface of the support in a sequence-independent manner in the presence of the detergent and absence of any chaotropic agent; and
(c) separating said magnetic particles with bound genomic DNA from said lysate. - View Dependent Claims (21)
- (b) producing a lysate by contacting said cells with a detergent and magnetic particles in the absence of any chaotropic agent, the magnetic particles comprising an organic polymer whereby soluble genomic DNA in said lysate is bound to the surface of the support in a sequence-independent manner in the presence of the detergent and absence of any chaotropic agent; and
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22. A method of isolating RNA and genomic DNA from cells in a sample, said method comprising (a) obtaining cells from said sample by immunomagnetic separation;
- (b) producing a lysate by contacting said cells with a detergent and magnetic particles in the absence of any chaotropic agent, the magnetic particles comprising an organic polymer, and whereby soluble genomic DNA in said lysate is bound to the surface of the support in a sequence-independent manner in the presence of the detergent and absence of any chaotropic agent;
(c) separating said magnetic particles with bound genomic DNA from said lysate; and
(d) isolating RNA from said lysate. - View Dependent Claims (23)
- (b) producing a lysate by contacting said cells with a detergent and magnetic particles in the absence of any chaotropic agent, the magnetic particles comprising an organic polymer, and whereby soluble genomic DNA in said lysate is bound to the surface of the support in a sequence-independent manner in the presence of the detergent and absence of any chaotropic agent;
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24. A method of isolating genomic DNA from a sample, said method comprising
(a) contacting said sample with a detergent and magnetic particles comprising an organic polymer wherein the surface of the magnetic particle has a negative, neutral or hydrophobic surface, and whereby soluble genomic DNA in said sample is bound to the surface of said particles in a sequence-independent manner; - and
(b) separating said magnetic particles with the bound genomic DNA from the sample.
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Specification