Stochastic scanning apparatus using multiphoton multifocal source
First Claim
Patent Images
1. A method of imaging comprising:
- illuminating a sample with light of wavelength n, wherein the light illuminates the sample over a random path across a sample region; and
using a detector to continuously collect a resulting radiation signal from the sample region.
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Abstract
A rapid-sampling stochastic scanning multiphoton multifocal microscopy (SS-MMM) fluorescence imaging technique enables multiparticle tracking at rates upwards of 1,000 times greater than conventional single point raster scanning. Stochastic scanning of a diffractive optical element may generate a 10×10 hexagonal array of foci with a white noise driven galvanometer to yield a scan pattern that is random yet space-filling. SS-MMM may create a more uniformly sampled image with fewer spatio-temporal artifacts than obtained by conventional or multibeam raster scanning.
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Citations
43 Claims
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1. A method of imaging comprising:
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illuminating a sample with light of wavelength n, wherein the light illuminates the sample over a random path across a sample region; and using a detector to continuously collect a resulting radiation signal from the sample region. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A method of imaging a sample, the method comprising:
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continuously randomly scanning a light beam as a plurality of foci light beams over a two-dimensional focal region at the sample; and using a detector to detect the resulting radiation signal from each of the foci light beams at the focal region of the sample. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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33. An apparatus for imaging a sample, the apparatus comprising:
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a light beam source producing a light beam comprising a plurality of spaced apart light beams; a scanner having an element disposed to receive the light beam and randomly scan the plurality of spaced apart light beams across a region of interest; and a focusing element disposed to focus the plurality of spaced apart light beams onto a focal region as a plurality of foci light beams; and a detector to detect the resulting radiation signal from the sample region. - View Dependent Claims (34, 35, 36, 37, 38, 39, 40, 41, 42, 43)
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Specification