Arrays, computer program products and methods for in silico array-based comparative binding assays
First Claim
1. An array for determining the relative amount of a biological molecule in a sample, the array comprising a plurality of biological molecules immobilized to a plurality of discrete and known spots on a substrate surface to form the array of biological molecules, wherein the array of spots comprises a plurality of test spots comprising different biological molecules and at least one calibration spot, and the at least one calibration spot comprises a mixture of the plurality of different biological molecules present in the test spots and wherein each of the discrete and known spots comprises a biological molecule that is a marker for each said spot on the array to provide a plurality of markers on the array.
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Accused Products
Abstract
The invention provides computer systems, computer program products and methods for in silico array-based methods for determining the relative amount of biological molecules (e.g., nucleic acid sequences) in two or more samples. The invention also provides novel arrays comprising immobilized calibration molecules (e.g., nucleic acids) for normalizing the results of array-based binding assays (e.g., hybridization reactions).
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Citations
38 Claims
- 1. An array for determining the relative amount of a biological molecule in a sample, the array comprising a plurality of biological molecules immobilized to a plurality of discrete and known spots on a substrate surface to form the array of biological molecules, wherein the array of spots comprises a plurality of test spots comprising different biological molecules and at least one calibration spot, and the at least one calibration spot comprises a mixture of the plurality of different biological molecules present in the test spots and wherein each of the discrete and known spots comprises a biological molecule that is a marker for each said spot on the array to provide a plurality of markers on the array.
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8. A system for performing comparative binding assays comprising:
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(a) an array comprising a first substrate surface comprising a first array and a second substrate surface comprising a second array, wherein the first and second arrays are separated by a hydrophobic barrier such that a first sample can be applied to the first array at the same time a second sample is applied to the second array without the two samples mixing together, and the first and the second arrays comprise the same calibration spots and wherein each of the first and second arrays comprise discrete and known spots comprising a nucleic acid sequence that is a marker for each said spot on the arrays to provide a plurality of markers on the arrays, and wherein at least one of the first and second arrays comprises test spots comprising different nucleic acid sequences, and wherein the calibration spots comprise a mixture of the different nucleic acid sequences from the test spots; and (b) a device for detecting a detectable label, wherein the device can measure which detectable labels are on which spots on the substrate surface. - View Dependent Claims (35)
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- 23. An array for determining the relative amount of a nucleic acid in a sample, the array comprising a plurality of nucleic acid molecules immobilized to a plurality of discrete and known spots on a substrate surface to form the array of nucleic acid molecules, wherein the array of spots comprises a plurality of test spots comprising different nucleic acids and at least one calibration spot, and the calibration spot comprises a mixture of the different nucleic acid sequences from the test spots and nucleic acid sequences from a genome other than that spotted on the array or in the sample, and wherein each of the discrete and known spots comprises a nucleic acid sequence that is a marker for each said spot on the array to provide a plurality of markers on the array.
- 33. A device comprising a first array and a second array respectively, the first and second array each comprising a plurality of nucleic acid segments, wherein each nucleic acid segment is immobilized to a discrete and known spot on each of a first substrate surface and a second substrate surface to form the first and the second arrays of nucleic acid segments wherein each said spot comprises a different nucleic acid sequence that is a marker for each said spot on the array to provide a plurality of markers on each array and the nucleic acid segments immobilized on the second array comprise substantially the same plurality of nucleic acid segments immobilized on the first array, each array further comprising at least one calibration spot comprising a mixture of the same plurality of nucleic acid sequence markers present in all of the other spots of the array.
Specification