Electrochemical analyte sensor
First Claim
1. A method of providing an electrochemical sensor, comprising:
- providing a substrate;
disposing conductive material on a surface of the substrate to form a plurality of traces, at least one of the traces forming a working electrode;
disposing an electron transfer agent proximate the working electrode; and
disposing an enzyme proximate to the working electrode;
wherein at least two of the conductive traces are separated on the surface of the substrate by 150 μ
m or less, the separation preventing conduction between the plurality of formed traces on the substrate; and
furtherwherein at least 90% of the electron transfer agent remains disposed on the substrate for at least 24 hours after subcutaneous implantation of the sensor in a body fluid.
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Accused Products
Abstract
An electrochemical analyte sensor having conductive traces on a substrate is used to determine a level of analyte in in vitro or in vivo analyte-containing fluids. The electrochemical analyte sensor includes a substrate and conductive material disposed on the substrate, the conductive material forming a working electrode. In some sensors, the conductive material is disposed in recessed channels formed in a surface of the sensor. An electron transfer agent and/or catalyst may be provided to facilitate the electrolysis of the analyte or of a second compound whose level depends on the level of the analyte. A potential is formed between the working electrode and a reference electrode or counter/reference electrode and the resulting current is a function of the concentration of the analyte in the fluid.
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Citations
22 Claims
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1. A method of providing an electrochemical sensor, comprising:
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providing a substrate; disposing conductive material on a surface of the substrate to form a plurality of traces, at least one of the traces forming a working electrode; disposing an electron transfer agent proximate the working electrode; and disposing an enzyme proximate to the working electrode; wherein at least two of the conductive traces are separated on the surface of the substrate by 150 μ
m or less, the separation preventing conduction between the plurality of formed traces on the substrate; and
furtherwherein at least 90% of the electron transfer agent remains disposed on the substrate for at least 24 hours after subcutaneous implantation of the sensor in a body fluid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method of providing an electrochemical sensor, comprising:
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providing a substrate; providing a conductive material disposed on a surface of the substrate to form a plurality of traces, at least one of the traces forming a working electrode; disposing an electron transfer agent proximate the working electrode; and disposing an enzyme proximate to the working electrode; wherein the plurality of conductive traces are disposed on the surface of the substrate at a density, along a width of the substrate, of about 150 to 700 μ
m/trace or less, each of the plurality of traces on the substrate separated by a predetermined distance to prevent conduction between the traces on the substrate; and
furtherwherein at least 90% of the electron transfer agent remains disposed on the substrate for at least 24 hours after subcutaneous implantation of the sensor in a body fluid. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22)
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Specification