User interface for a flow cytometer system
First Claim
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1. A method of extracting and analyzing a data set from a flow cytometer system comprising:
- collecting a full dynamic range of input signals from a flow cytometer sample;
storing an initial data set of the full dynamic range of the input signals from the flow cytometer sample;
displaying the initial data set on a user interface;
allowing modification of the initial data set to manipulate the initial data set across the range of input signals from the flow cytometer sample;
saving the modified data set; and
exporting the saved data set to a different medium than the flow cytometer system;
wherein collecting the full dynamic range of input signals includes collecting the full dynamic range of input signals to an initial data set that includes a 1;
100,000 ratio between the faintest objects and the brightest objects.
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Abstract
A method of extracting and analyzing a data set from a flow cytometer system of the preferred embodiment comprises the steps of (1) running a sample and saving all collected raw data, (2) viewing raw (or “unmodified”) data, (3) modifying the raw data (e.g., scaling and/or culling the raw data), (4) reviewing and saving the modified data, and (5) exporting the saved data. Once the sample has been run and all collected data have been saved, the user can repeat the steps of modifying the raw data, saving the modified data, and exporting the saved data as many times as necessary and/or desirable.
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Citations
36 Claims
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1. A method of extracting and analyzing a data set from a flow cytometer system comprising:
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collecting a full dynamic range of input signals from a flow cytometer sample; storing an initial data set of the full dynamic range of the input signals from the flow cytometer sample; displaying the initial data set on a user interface; allowing modification of the initial data set to manipulate the initial data set across the range of input signals from the flow cytometer sample; saving the modified data set; and exporting the saved data set to a different medium than the flow cytometer system; wherein collecting the full dynamic range of input signals includes collecting the full dynamic range of input signals to an initial data set that includes a 1;
100,000 ratio between the faintest objects and the brightest objects.- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method of extracting and analyzing a data set from a flow cytometer system comprising:
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collecting a full dynamic range of input signals from a flow cytometer sample; storing an initial data set of the full dynamic range of the input signals from the flow cytometer sample; displaying the initial data set on a user interface; allowing modification of the initial data set to manipulate the initial data set across the range of input signals from the flow cytometer sample; saving the modified data set; and exporting the saved data set to a different medium than the flow cytometer system; wherein allowing modification of the initial data set includes permitting a user to manipulate the initial data set across the full dynamic range of input signals from the flow cytometer sample and to generate a modified data set, wherein allowing modification of the initial data set further includes permitting the user to; perform real-time comparisons between the initial data set and the modified data set on a single flow cytometer sample, adjust or undo modifications, to make multiple adjustments on the same initial data set, and generate at least one subset of data that corresponds to one or more sample populations contained within the initial data set. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22)
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23. A method of extracting and analyzing a data set from a flow cytometer system comprising:
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collecting a full dynamic range of input signals from a flow cytometer sample; storing an initial data set of the full dynamic range of the input signals from the flow cytometer sample; displaying the initial data set on a user interface; allowing modification of the initial data set to manipulate the initial data set across the range of input signals from the flow cytometer sample; saving the modified data set; and exporting the saved data set to a different medium than the flow cytometer system; wherein allowing modification of the initial data set further includes permitting a user to identify the modifications made to the initial data set and to store settings used to generate a desired subset of data. - View Dependent Claims (24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36)
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Specification