Biosensor

• US 7,998,336 B2
• Filed: 01/31/2008
• Issued: 08/16/2011
• Est. Priority Date: 09/12/1997
• Status: Expired due to Fees

• Current Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories)
• Original Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories)
• Inventors: Mure, Hiroki, Shirakawa, Hiroshi, Gotoh, Masao
• Primary Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US12/068,014
• Publication Number:
  US 20080251377A1
• Time in Patent Office: 1,293 Days
• Field of Search: 20440301-40315, 205/777.5, 205/778, 205/792
• US Class Current: 205/777.5
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

A biosensor comprises a space part for sucking and housing a sample formed of two upper and lower plates, the two plates being stuck together by an adhesive layer, the space part for sucking and housing the sample being constituted so as to be partially opened in the peripheral part and partially closed by the adhesive layer, and has a working electrode having at least glucose oxidase immobilized thereon and a counter electrode on the same plane of the plate.

Claims

1. A biosensor comprising:

a proximal end and a distal end;

a first layer comprising a first side edge having a first length and a second side edge opposite to the first side edge and having a second length, wherein the first and second lengths are different;

a second layer comprising a first side edge having a first length and a second side edge opposite to the first side edge and having a second length, wherein the first and second lengths are different;

the first and second layers fixed together by a third layer;

the proximal end having a tapered part;

a working electrode provided on the first layer and a counter electrode provided on the second layer, wherein the tip part of the working electrode is provided on the tapered part, and wherein the leads from the working and counter electrodes extend from the tapered part to the distal end,

the biosensor further comprising a reference electrode and a tip part of the reference electrode is provided on the tapered part.

2. The biosensor of claim 1, wherein the tapered part includes an opening for admitting sample.

3. The biosensor of claim 2, further comprising a space for housing a sample and the opening is in communication with the space for housing the sample.

4. The biosensor of claim 3, wherein the space has a volume in the range of about 0.5-10 microliters.

5. The biosensor of claim 3, wherein a tip part of the counter electrode is provided on the tapered part.

6. The biosensor of claim 1, wherein the third layer comprises an adhesive.

7. The biosensor of claim 1, wherein the third layer comprises a first side edge of a third length, wherein the third length is different from the first and second lengths.

8. The biosensor of claim 1, further comprising an oxidoreductase.

9. The biosensor of claim 8, wherein the oxidoreductase is selected from the group of glucose oxidase and glucose dehydrogenase.

10. The biosensor of claim 1, wherein the biosensor is connectable to a device body having a control part including a display part, and the biosensor further comprises an insertion judging electrode for interconnecting two input terminals of the device body to start the control part of the device body upon connecting the biosensor to the device body.

11. A method of measuring a concentration of an analyte in a subject, the method comprising:

contacting body fluid of the subject with a biosensor to generate an electrical signal, the biosensor comprising;

a proximal end and a distal end;

a first layer comprising a first side edge having a first length and a second side edge opposite to the first side edge and having a second length, wherein the first and second lengths are different;

a second layer comprising a first side edge having a first length and a second side edge opposite to the first side edge and having a second length, wherein the first and second lengths are different;

the first and second layers fixed together by a third layer;

the proximal end having a tapered part;

a working electrode provided on the first layer and a counter electrode provided on the second layer, wherein the tip part of the working electrode is provided on the tapered part, and wherein the leads from the working and counter electrodes extend from the tapered part to the distal end,

the biosensor further comprising a reference electrode and a tip part of the reference electrode is provided on the tapered part; and

determining from the generated electrical signal a concentration of an analyte in body fluid.

12. The method of measuring a concentration of an analyte in the subject as claimed in claim 11, wherein the contacting comprises contacting the tapered part of the biosensor to the body fluid.

13. The method of measuring a concentration of an analyte in the subject as claimed in claim 11, wherein the tapered part comprises an opening in communication with a space for housing the body fluid, and the method comprises admitting the body fluid into the space through the opening in the tapered part.

14. The method of measuring a concentration of an analyte in the subject as claimed in claim 11, wherein the body fluid ranges from about 0.5 to about 10 microliters.

15. The method of measuring a concentration of an analyte in the subject as claimed in claim 11, wherein the biosensor comprises an oxidoreductase.

16. The method of measuring a concentration of an analyte in the subject as claimed in claim 15, wherein the oxidoreductase is selected from the group of glucose oxidase and glucose dehydrogenase.

17. The method of measuring a concentration of an analyte in the subject as claimed in claim 11, wherein the biosensor is connectable to a device body having a control part including a display part and the biosensor further comprises a biosensor insertion signal terminal for interconnecting two input terminals of the device body to start the control part of the device body upon connecting the biosensor to the device body.