High density plate filler

• US 7,998,435 B2
• Filed: 03/30/2006
• Issued: 08/16/2011
• Est. Priority Date: 09/19/2003
• Status: Active Grant

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Reed, Mark T.
• Primary Examiner(s): Warden; Jill
• Assistant Examiner(s): Handy; Dwayne K
• Application Number:
  US11/393,061
• Publication Number:
  US 20060233672A1
• Time in Patent Office: 1,965 Days
• Field of Search: 422100-104, 422/501, 422/502, 422/503, 422/504, 422/507, 436/180
• US Class Current: 422/507
• CPC Class Codes: B01L 2200/021 Adjust spacings in an array of wells, pipettes or holders, format transfer between arrays of different size or geometry
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/0605 Metering of fluids
• CPC Class Codes: B01L 2200/0642 Filling fluids into wells by specific techniques
• CPC Class Codes: B01L 2200/0684 Venting, avoiding backpressure, avoid gas bubbles
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/1805 Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/0293 for liquids
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/50851 specially adapted for heating or cooling samples laboratory heating apparatus B01L7/00; incubators C12M
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: B01L 3/563 Joints or fittings in general F16L; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors
• CPC Class Codes: G01N 35/1074 arranged in a two-dimensional array
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A filling apparatus for filling a microplate. The microplate having a plurality of wells each sized to receive an assay. The filling apparatus can comprise an output layer having a plurality of capillaries, wherein a first grouping of the capillaries is separated from a second grouping by a hydrophobic feature. Each of the plurality of capillaries can comprise an inlet and an outlet. A funnel assembly can comprise a funnel member sized to receive the assay. The funnel member can comprise an outlet for delivering a fluid bead of the assay along a top surface of the output layer and in fluid communication with each of the plurality of capillaries such that a portion of the fluid bead can be drawn within at least some of the plurality of capillaries in response to capillary force. The funnel assembly and the output layer can be moveable relative to each other between a first position and a second position to draw the fluid bead across the top surface.

Claims

1. A filling system for receiving a sample comprising:

an output layer having a plurality of capillaries, each of said plurality of capillaries having an inlet and an outlet, said output layer having at least one hydrophobic feature disposed thereon separating a first grouping of said plurality of capillaries from a second grouping of said plurality of capillaries; and

a surface wipe assembly comprising;

a base supporting said output layer; and

a funnel assembly engaging said base and moveable relative to said base, said funnel assembly having a funnel member sized to receive said sample, said funnel member having an outlet for delivering a fluid bead of said sample along a top surface of said output layer and in fluid communication with each of said plurality of capillaries such that a portion of said fluid bead is drawn within at least some of the plurality of capillaries in response to capillary force, said funnel assembly and said output layer being moveable relative to each other between a first position and a second position to draw said fluid bead across said top surface.

2. The filling system according to claim 1 wherein said at least one hydrophobic feature is a line extending substantially from one end of said output layer to an opposing end.

3. The filling system according to claim 1 wherein said hydrophobic feature is disposed between adjacent rows of said plurality of capillaries.

4. The filling system according to claim 1 wherein said hydrophobic feature is disposed along a row of said plurality of capillaries.

5. The filling system according to claim 1 wherein said at least one hydrophobic feature is formed on said output layer through at least one of pad printing, silk screening, and plasma coating.

6. The filling system according to claim 1 wherein said at least one hydrophobic feature is made of at least one of PDMS, silicone, Teflon, and paralene.

7. The filling system according to claim 1, further comprising:

a drive assembly operably coupled to one of said output layer and said funnel assembly to provide said relative movement of said funnel assembly and said output layer between said first position and said second position.

8. The filling system according to claim 7 wherein said drive assembly comprises:

a drive motor operably coupled to one of said output layer and said funnel assembly; and

a control system operably coupled to said drive motor, said control system selectively actuating said drive motor in response to a control input.

9. The filling system according to claim 8 wherein said drive assembly further comprises:

said base support for supporting said output layer;

a guide member extending from said base support; and

a tracking member extending from said funnel assembly, said tracking member engagable with said guide member to guide said funnel assembly relative to said output layer.

10. The filling system according to claim 9 wherein said funnel assembly comprises:

a spanning portion having a slot formed therethrough;

a funnel member disposed in said slot.

11. The filling system according to claim 1 wherein said output layer comprises:

an overflow channel formed therein, said overflow channel being in fluid communication with at least a portion of said top surface of said output layer.

12. The filling system according to claim 1 wherein said funnel assembly comprises:

a funnel member having a first sample chamber and a second sample chamber, said first sample chamber being in fluid communication with said first grouping of said plurality of capillaries and said second sample chamber being in fluid communication with said second grouping of said plurality of capillaries.

13. The filling system according to claim 1 wherein each of said plurality of capillaries is sized to provide sufficient force to draw a predetermined volume of said sample therein and permit release of said sample from each of said plurality of capillaries in response to an applied centripetal force.

27. A method of distributing a sample comprising:

providing the device of claim 1; and

drawing a first fluid bead of a first sample across said output layer such that at least a portion of said first fluid bead is drawn within said first grouping of said plurality of capillaries.

28. The method according to claim 27 further comprising:

drawing a second fluid bead of a second sample across said output layer such that at least a portion of said second fluid bead is drawn within a second of said plurality of capillaries.

14. A filling system for receiving a sample comprising:

an output layer having a plurality of capillaries, each of said plurality of capillaries having an inlet and an outlet, said output layer having at least one hydrophobic feature separating a first grouping of said plurality of capillaries from a second grouping of said plurality of capillaries; and

a surface wipe assembly comprising;

a base supporting said output layer; and

a funnel assembly engaging said base and moveable relative to said base, said funnel assembly having a first sample chamber and a second sample chamber, said first sample chamber being in fluid communication with said first grouping of said plurality of capillaries and said second sample chamber being in fluid communication with said second grouping of said plurality of capillaries, said first grouping being separate from said second grouping, said first sample chamber and said second sample chamber delivering separate fluid beads of said sample along said top surface of said output layer and in fluid communication with said first grouping and said second grouping respectively to draw at least a portion of said fluid bead in to at least some of the plurality of capillaries in response to capillary force.

15. The filling system according to claim 14 wherein said at least one hydrophobic feature is a line extending substantially from one end of said output layer to an opposing end.

16. The filling system according to claim 14 wherein said hydrophobic feature is disposed between adjacent rows of said plurality of capillaries.

17. The filling system according to claim 14 wherein said hydrophobic feature is disposed along a row of said plurality of capillaries.

18. The filling system according to claim 14 wherein said at least one hydrophobic feature is formed on said output layer through at least one of pad printing, silk screening, and plasma coating.

19. The filling system according to claim 14 wherein said at least one hydrophobic feature is made of at least one of PDMS, silicone, Teflon, and paralene.

20. The filling system according to claim 14, further comprising:

a drive assembly operably coupled to one of said output layer and said funnel assembly to provide relative movement of said funnel assembly and said output layer between a first position and a second position.

21. The filling system according to claim 15 wherein said drive assembly comprises:

a drive motor operably coupled to one of said output layer and said funnel assembly; and

a control system operably coupled to said drive motor, said control system selectively actuating said drive motor in response to a control input.

22. The filling system according to claim 21 wherein said drive assembly further comprises:

said base support for supporting said output layer;

a guide member extending from said base support; and

a tracking member extending from said funnel assembly, said tracking member engagable with said guide member to guide said funnel assembly relative to said output layer.

23. The filling system according to claim 22 wherein said funnel assembly comprises:

a spanning portion having a slot formed therethrough;

a funnel member disposed in said slot.

24. The filling system according to claim 20 wherein said output layer comprises:

an overflow channel formed therein, said overflow channel being in fluid communication with at least a portion of said top surface of said output layer.

25. The filling system according to claim 24 wherein said funnel assembly comprises:

a funnel member having a first sample chamber and a second sample chamber, said first sample chamber being in fluid communication with said first grouping of said plurality of capillaries and said second sample chamber being in fluid communication with said second grouping of said plurality of capillaries.

26. The filling system according to claim 20 wherein each of said plurality of capillaries is sized to provide sufficient force to draw a predetermined volume of said sample therein and permit release of said sample from each of said plurality of capillaries in response to an applied centripetal force.