Microfluidic assembly with coupled microfluidic devices

• US 7,998,437 B2
• Filed: 02/16/2007
• Issued: 08/16/2011
• Est. Priority Date: 08/18/2004
• Status: Active Grant

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Berndt, Manfred, Gassmann, Marcus
• Primary Examiner(s): Bullock; In Suk
• Assistant Examiner(s): Kingan; Timothy G
• Application Number:
  US11/707,429
• Publication Number:
  US 20070154355A1
• Time in Patent Office: 1,642 Days
• Field of Search: 422/400, 422500-544
• US Class Current: 422/539
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0874 Three dimensional network
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/0421 electrophoretic flow
• CPC Class Codes: B01L 2400/049 vacuum
• CPC Class Codes: B01L 2400/065 sliding valves
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027

Abstract

A microfluidic assembly (1;57) has at least one microfluidic flow path (17,27,29,35,38;82,85,95) and at least one inlet port (11,13,15;81,97,101) coupled to the flow path (17,27,29,35,38;82,85,95). The microfluidic assembly (1;57) has a first microfluidic device (7;63) that executes a microfluidic process and has a second microfluidic device (9;61). The microfluidic assembly (1;57) has an interface (5;65). The interface (5;65) couples the first microfluidic device (7;63) and the second microfluidic device (9;61).

Claims

1. A microfluidic assembly comprising:

two microfluidic devices, wherein at least one of the microfluidic devices is configured for executing a microfluidic process,

wherein one of the two microfluidic devices comprises a valve, coupled to the other microfluidic device, the valve comprising a structure layer interposed between a bottom layer and a top layer, wherein the structure layer comprises a planar substrate having a first flow path that selectively connects a plurality of second flow path formed therein that selectively connects a plurality of second flow paths between the two microfluidic devices.

2. The assembly of claim 1, wherein the valve is configured to control a flow within the at least flow path by selectively connecting and/or disconnecting the at least one flow path.

3. The assembly of claim 1, wherein the structure layer comprises an optional-route slider.

4. The assembly of claim 1, wherein the structure layer comprises a slider configured for at least one of sliding relative to, sliding rectilinearly to, or rotating relatively to, at least one of the microfluidic devices.

5. The assembly of claim 1, wherein the valve comprises at least one microfluidic control element for controlling a flow within the at least one flow path.

6. The assembly of claim 1, wherein at least one of the microfluidic devices comprises an optically transmissible material.

7. The assembly of claim 1, wherein the valve is an integrated part of one of the microfluidic devices.

8. The assembly of claim 1, wherein a first one of the microfluidic devices is adapted for executing a first microfluidic process, and wherein a second one of the microfluidic devices is adapted for executing a second microfluidic process.

9. A microfluidic assembly comprising:

two microfiuidic devices, wherein at least one of the microfluidic devices is configured for executing a microfluidic process,

wherein one of the two microfluidic devices comprises a valve, coupled to the other microfluidic device, the valve comprising a structure layer interposed between a bottom layer and a top layer, wherein the structure layer is configured to provide at least one selectively connectable flow path between the two microfluidic devices,

wherein the structure layer comprises a slider configured for at least one of sliding relative to, sliding rectilincarly to, or rotating relatively to, at least one of the microfluidic devices.

10. Method of preparing and analyzing a sample with a microfluidic assembly, comprising:

loading a sample to an inlet port of the microfluidic assembly,

executing a first biochemical process within a first microfluidic device of the microfluidic assembly, and

transporting at least part of the sample from the first to a second microfluidic device of the microfluidic assembly via a structure layer interposed between a bottom layer and a top layer of a valve incorporated in one of the first and second microfluidic devices, before, while, or after executing the first biochemical process.

11. Method of claim 10, further comprising:

transporting at least part of the sample to at least one of an analytical area, an electrophoresis channel and a detection area, of the microfluidic assembly.

12. Method of claim 10, further comprising:

changing a setting of the valve after executing the first biochemical process.

13. Method of claim 10, further comprising:

bringing the valve of the microfluidic assembly to a first setting,

loading a sample in a sample inlet port of the microfluidic assembly,

bringing the sample in contact with a binding and/or adsorbing material,

bringing the valve to a second setting,

loading a processing or washing reagent to a processing reagent port of the microfluidic assembly,

bringing the valve to a third position,

loading an elution solvent to a sample elution port of the microfluidic assembly, and

eluting the sample.

14. Method of claim 13, further comprising:

loading the ports by applying a vacuum to a vacuum port of the microfluidic assembly, by applying the according liquid under pressure to the ports, or by applying a voltage.

15. Method of claim 10, further comprising:

closing the valve of the microfluidic assembly after loading the sample to the inlet port of the microfluidic assembly,

executing at least one PCR-cycle within the microfluidic assembly,

opening the valve of the microfluidic assembly before transporting at least part of the sample from the first microfluidic device to the second microfluidic device via the structure layer.

16. Method of claim 15, further comprising:

loading a sample with different sample contents to be processed to the inlet port.

17. Method of claim 10, further comprising:

opening the valve after each cycle of the PCR-reaction for transporting part of the sample and for monitoring the complete PCR-process.

18. Method of claim 10, further comprising:

executing a hybridization process of the sample within the microfluidic assembly after loading the sample to the inlet port of the microfluidic assembly.

19. Method of claim 10, further comprising:

executing a first microfluidic process within the structure layer,

executing a second microfluidic process within the second microfluidic device.