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Microfluidic system for amplifying and detecting polynucleotides in parallel

DC CAFC
  • US 7,998,708 B2
  • Filed: 11/14/2007
  • Issued: 08/16/2011
  • Est. Priority Date: 03/24/2006
  • Status: Active Grant
First Claim
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1. An apparatus, comprising:

  • a multi-lane microfluidic cartridge, each lane comprising a PCR reaction zone;

    a receiving bay configured to receive the microfluidic cartridge;

    each PCR reaction zone comprising a separately controllable heat source thermally coupled thereto, wherein the heat source maintains a substantially uniform temperature throughout the PCR reaction zone and thermal cycles the PCR reaction zone to carry out PCR on a polynucleotide-containing sample in the PCR reaction zone;

    a detector configured to detect the presence of an amplification product in the respective PCR reaction zone; and

    a processor coupled to the detector and the heat source, configured to control heating of one or more PCR reaction zones by the heat sources.

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