Multi-channel swept wavelength optical interrogation system and method for using same
First Claim
1. An optical interrogation system comprising:
- a tunable laser that emits an optical beam which has a predetermined sequence of distinct wavelengths over a predetermined time period;
a distribution unit that splits the optical beam into a plurality of interrogation beams;
an array of optical interrogation units that receive and direct the interrogation beams towards an array of biosensors, wherein each of the optical interrogation units receives one of the interrogation beams and directs the one interrogation beam to one of the biosensors, wherein each biosensor has a signal area and a reference area, and wherein each optical interrogation unit further includes a photo detector with two photodiodes;
said array of optical interrogation units receive a plurality of reflected interrogation beams from the array of biosensors, wherein each of the optical interrogation units receives a directed one interrogation beam as a reflected interrogation beam from one of the biosensors;
a data processing device that receives and processes information associated with the reflected interrogation beams; and
a holder that supports the array of biosensors where said holder includes an array of masks, wherein each of the masks blocks a portion of one of the biosensors from being illuminated by one of the interrogation beams, wherein the portion is a central portion between the signal area and the reference area of the one biosensor, and wherein each mask blocks one of the reflected interrogation beams off the corresponding one biosensor from illuminating a gap between the two photodiodes of the photo detectors of the corresponding optical interrogation unit.
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Accused Products
Abstract
A multi-channel swept wavelength optical interrogation system and a method are described herein that enable the interrogation of one or more biosensors which for example could be located within the wells of a microplate. In one embodiment, the optical interrogation system comprises: (a) a tunable laser that emits an optical beam which has a predetermined sequence of distinct wavelengths over a predetermined time period; (b) a distribution unit that splits the optical beam into a plurality of interrogation beams; (c) an array of optical interrogation units that receive and direct the interrogation beams towards an array of biosensors; (d) the array of optical interrogation units receive a plurality of reflected interrogation beams from the array of biosensors; (e) a data processing device that receives and processes information associated with the reflected interrogation beams to determine for example whether or not there was a biochemical interaction on anyone of the biosensors.
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Citations
21 Claims
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1. An optical interrogation system comprising:
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a tunable laser that emits an optical beam which has a predetermined sequence of distinct wavelengths over a predetermined time period; a distribution unit that splits the optical beam into a plurality of interrogation beams; an array of optical interrogation units that receive and direct the interrogation beams towards an array of biosensors, wherein each of the optical interrogation units receives one of the interrogation beams and directs the one interrogation beam to one of the biosensors, wherein each biosensor has a signal area and a reference area, and wherein each optical interrogation unit further includes a photo detector with two photodiodes; said array of optical interrogation units receive a plurality of reflected interrogation beams from the array of biosensors, wherein each of the optical interrogation units receives a directed one interrogation beam as a reflected interrogation beam from one of the biosensors; a data processing device that receives and processes information associated with the reflected interrogation beams; and a holder that supports the array of biosensors where said holder includes an array of masks, wherein each of the masks blocks a portion of one of the biosensors from being illuminated by one of the interrogation beams, wherein the portion is a central portion between the signal area and the reference area of the one biosensor, and wherein each mask blocks one of the reflected interrogation beams off the corresponding one biosensor from illuminating a gap between the two photodiodes of the photo detectors of the corresponding optical interrogation unit. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. An optical interrogation system comprising:
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a tunable laser that emits an optical beam which has a predetermined sequence of distinct wavelengths over a predetermined time period; a distribution unit that splits the optical beam into a plurality of interrogation beams; an array of optical interrogation units that receive and direct the interrogation beams towards an array of biosensors, wherein each of the optical interrogation units receives one of the interrogation beams and directs the one interrogation beam to one of the biosensors; said array of optical interrogation units receive a plurality of reflected interrogation beams from the array of biosensors, wherein each of the optical interrogation units receives a directed one interrogation beam as a reflected interrogation beam from one of the biosensors; and a data processing device that receives and processes information associated with the reflected interrogation beams, wherein said data processing device further includes a memory that stores processor-executable instructions and a processor that interfaces with the memory and executes the processor-executable instructions to; acquire interrogation data obtained by a plurality of photo detectors in said plurality of optical interrogation units where said plurality of photo detectors receive the plurality of reflected interrogation beams from the array of bio sensors; acquire coarse wavelength data and fine wavelength data from a wavelength tracking device; acquire power data from a power tracking device which tracks the power of said tunable laser; track distinct wavelengths of the optical beam emitted from said tunable laser using the coarse wavelength data and the fine wavelength data; find instants of zero-crossings of fringes associated with the fine wavelength data; integrate the interrogation data for each biosensor that was received between trigger points which correspond to adjacent instants of the zero-crossings of the fringes associated with the fine wavelength data; normalize the integrated interrogation data using the power data from the power tracking device; digitally filter the normalized integrated interrogation data; and calculate a centroid for each digitally filtered normalized integrated interrogation data to determine a resonant wavelength for each of the biosensors. - View Dependent Claims (14)
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15. An optical interrogation system comprising:
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a tunable laser that emits an optical beam which has a predetermined sequence of distinct wavelengths over a predetermined time period; a distribution unit that splits the optical beam into a plurality of interrogation beams; an array of optical interrogation units that receive and direct the interrogation beams towards an array of biosensors, wherein each of the optical interrogation units receives one of the interrogation beams and directs the one interrogation beam to one of the biosensors; said array of optical interrogation units receive a plurality of reflected interrogation beams from the array of biosensors, wherein each of the optical interrogation units receives a directed one interrogation beam as a reflected interrogation beam from one of the biosensors; and a data processing device that receives and processes information associated with the reflected interrogation beams, wherein said data processing device further includes a field programmable gate array, FPGA, processing board or an application specific integrated circuit, ASIC, which is used to; acquire interrogation data obtained by a plurality of photo detectors in said plurality of optical interrogation units where said plurality of photo detectors receive the plurality of reflected interrogation beams from the array of bio sensors; acquire coarse wavelength data and fine wavelength data from a wavelength tracking device; acquire power data from a power tracking device which tracks the power of said tunable laser; track distinct wavelengths of the optical beam emitted from said tunable laser using the coarse wavelength data and the fine wavelength data; generate a trigger point at each instant when a fringe associated with the fine wavelength data crosses an average level where the average level is determined by using a minimum level and a maximum level of a previous fringe period to compute a predicted average level and then comparing a level of a current fringe with the predicted average level; integrate the interrogation data for each biosensor that was received between each pair of trigger points which are associated with the fine wavelength data; and normalize the integrated interrogation data using the power data from the power tracking device; said data processing device further includes a memory that stores processor-executable instructions and a processor that interfaces with the memory and executes the processor-executable instructions to; digitally filter the normalized integrated interrogation data; and calculate a centroid for each digitally filtered normalized integrated interrogation data to determine a resonant wavelength for each of the biosensors. - View Dependent Claims (16)
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17. A method for interrogating one or more biosensors, said method comprising the steps of:
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emitting, from a tunable laser, an optical beam which has a predetermined sequence of distinct wavelengths over a predetermined time period; splitting, at a distribution unit, the optical beam into one or more interrogation beams; directing, from an array of optical interrogation units, the one or more interrogation beams towards one or more biosensors, wherein each of the optical interrogation units receives one of the interrogation beams and directs the one interrogation beam to one of the biosensors, wherein each biosensor has a signal area and a reference area, and wherein each optical interrogation unit further includes a photo detector with two photodiodes; receiving, at the array of optical interrogation units, one or more reflected interrogation beams from the one or more biosensors, wherein each of the optical interrogation units receives a directed one interrogation beam as a reflected interrogation beam from one of the biosensors; processing information associated with the reflected interrogation beams; and said array of biosensors are supported by a holder which includes an array of masks, wherein each of the masks blocks a portion of one of the biosensors from being illuminated by one of the interrogation beams, wherein the portion is a central portion between the signal area and the reference area of the one biosensor, and wherein each mask blocks one of the reflected interrogation beams off the corresponding one biosensor from illuminating a gap between the two photodiodes of the photo detectors of the corresponding optical interrogation unit. - View Dependent Claims (18, 21)
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19. A method for interrogating one or more biosensors, said method comprising the steps of:
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emitting, from a tunable laser, an optical beam which has a predetermined sequence of distinct wavelengths over a predetermined time period; splitting, at a distribution unit, the optical beam into one or more interrogation beams; directing, from an array of optical interrogation units, the one or more interrogation beams towards one or more biosensors, wherein each of the optical interrogation units receives one of the interrogation beams and directs the one interrogation beam to one of the biosensors, wherein each biosensor has a signal area and a reference area, and wherein each optical interrogation unit further includes a photo detector with two photodiodes; receiving, at the array of optical interrogation units, one or more reflected interrogation beams from the one or more biosensors, wherein each of the optical interrogation units receives a directed one interrogation beam as a reflected interrogation beam from one of the biosensors; processing information associated with the reflected interrogation beams;
wherein said step of processing further includes the steps of;acquiring interrogation data obtained by one or more photo detectors which receive the one or more reflected interrogation beams from the one or more biosensors; acquiring coarse wavelength data and fine wavelength data from a wavelength tracking device; acquiring power data from a power tracking device which tracks the power of the emitted optical beam; tracking distinct wavelengths of the emitted optical beam using the coarse wavelength data and the fine wavelength data; finding instants of zero-crossings of fringes associated with the fine wavelength data;
integrating the interrogation data for each biosensor that was received between triggerpoints which correspond to adjacent instants of the zero-crossings of the fringes associated with the fine wavelength data; normalizing the integrated interrogation data using the power data from the power tracking device; digitally filtering the normalized integrated interrogation data; and calculating a centroid for each digitally filtered normalized integrated interrogation data to determine a resonant wavelength for the one or more biosensors.
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20. A method for interrogating one or more biosensors, said method comprising the steps of:
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emitting, from a tunable laser, an optical beam which has a predetermined sequence of distinct wavelengths over a predetermined time period; splitting, at a distribution unit, the optical beam into one or more interrogation beams; directing, from an array of optical interrogation units, the one or more interrogation beams towards one or more biosensors, wherein each of the optical interrogation units receives one of the interrogation beams and directs the one interrogation beam to one of the biosensors, wherein each biosensor has a signal area and a reference area, and wherein each optical interrogation unit further includes a photo detector with two photodiodes; receiving, at the array of optical interrogation units, one or more reflected interrogation beams from the one or more biosensors, wherein each of the optical interrogation units receives a directed one interrogation beam as a reflected interrogation beam from one of the biosensors; processing information associated with the reflected interrogation beams;
wherein said step of processing further includes the steps of;acquiring interrogation data obtained by one or more photo detectors which receive the one or more reflected interrogation beams from the one or more biosensors; acquiring coarse wavelength data and fine wavelength data from a wavelength tracking device; acquiring power data from a power tracking device which tracks power of the emitted optical beam; tracking distinct wavelengths of the emitted optical beam using the coarse wavelength data and the fine wavelength data; generating a trigger point at each instant when a fringe associated with the fine wavelength data crosses an average level where the average level is determined by using a minimum level and a maximum level of a previous fringe period to compute a predicted average level and then comparing a level of a current fringe with the predicted average level; integrating the interrogation data for each biosensor that was received between each pair of trigger points which are associated with the fine wavelength data; and normalizing the integrated interrogation data using the power data from the power tracking device; digitally filtering the normalized integrated interrogation data; and calculating a centroid for each digitally filtered normalized integrated interrogation data to determine a resonant wavelength for the one or more biosensors.
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Specification