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Reaction vessel with integrated optical and fluid control elements

  • US 8,003,060 B2
  • Filed: 05/16/2008
  • Issued: 08/23/2011
  • Est. Priority Date: 05/18/2007
  • Status: Expired due to Fees
First Claim
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1. A disposable reaction vessel, comprising:

  • a) a housing including a first housing section and a second housing section form fitted to said first housing section so that when the first and second housing sections are assembled to form said housing they are in sealing relationship;

    b) a reaction chamber located inside of said housing;

    c) an optical element integrally formed on an outside surface of the second housing section and located adjacent to said reaction chamber on the inside of the housing, said second housing section being at least partially constructed of a material which will transmit light to and from the reaction chamber;

    d) a waste reservoir located on an interior of said housing,e) a first flow passageway between said reaction chamber and said waste reservoir;

    f) reagent and sample inlets located at a first end portion of said housing;

    g) a second flow passageway between said reagent and sample inlets and said reaction chamber;

    h) an end cap having a size and shape to slide over said first end portion and snap fit thereon to be locked onto said housing and form a leak tight seal, wherein said end cap includes at least one liquid reservoir for holding reagents and/or liquid sample, said at least one liquid reservoir including pierceable sealing means, and wherein said reagent and sample inlets pierce said pierceable sealing means when said end cap is slid onto said housing;

    i) a pattern of analyte-specific receptors bound to an inner surface of the reaction chamber, the patterned area being substantially surrounded by an array of radially oriented microfluidic channels configured to direct fluid from said reaction chamber radially outward to said first flow passageway; and

    j) said optical element directs a light beam to the reaction chamber for interrogating the pattern of analyte specific receptors, wherein the light beam interacts with the pre-selected pattern of analyte specific receptors and analytes bound thereto, and wherein the light beam that interacts with the pre-selected pattern of analyte-specific receptors and analytes bound thereto is a diffracted light beam which is directed away from said inner surface by said optical element.

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