Reaction vessel with integrated optical and fluid control elements

• US 8,003,060 B2
• Filed: 05/16/2008
• Issued: 08/23/2011
• Est. Priority Date: 05/18/2007
• Status: Expired due to Fees

• Current Assignee: Angle Europe Limited (ANGLE Plc)
• Original Assignee: Axela Inc.
• Inventors: Liederman, Adam Brian, Ganske, Rocky, Cracauer, Raymond Francis
• Primary Examiner(s): Warden; Jill
• Assistant Examiner(s): Woodard; Joye L
• Application Number:
  US12/153,366
• Publication Number:
  US 20080286858A1
• Time in Patent Office: 1,194 Days
• Field of Search: 422/57, 422/58, 422/61, 422/82.05, 422/102, 422/104, 422/404, 422/413, 422/430, 422/503, 422/512, 422/570, 435/287.6, 435/288.7
• US Class Current: 422/413
• CPC Class Codes: B01L 2200/04 Exchange or ejection of cartridges, containers or reservoirs
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/022 Transponder chips
• CPC Class Codes: B01L 2300/0809 rectangular shaped
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00

Abstract

The present invention provides disposable, semi-reusable, or single use reaction vessels with integrated optical elements for use with diffraction based assay systems. The vessel for assaying liquids for analytes includes a housing having at least one chamber or well for receiving a liquid therein and an optical element integrally formed with the housing for directing an incident light beam towards the well or chamber and directing a light beam away from the reaction chamber after the light beam has interacted with analytes present in the liquid.

Claims

1. A disposable reaction vessel, comprising:

a) a housing including a first housing section and a second housing section form fitted to said first housing section so that when the first and second housing sections are assembled to form said housing they are in sealing relationship;

b) a reaction chamber located inside of said housing;

c) an optical element integrally formed on an outside surface of the second housing section and located adjacent to said reaction chamber on the inside of the housing, said second housing section being at least partially constructed of a material which will transmit light to and from the reaction chamber;

d) a waste reservoir located on an interior of said housing,

e) a first flow passageway between said reaction chamber and said waste reservoir;

f) reagent and sample inlets located at a first end portion of said housing;

g) a second flow passageway between said reagent and sample inlets and said reaction chamber;

h) an end cap having a size and shape to slide over said first end portion and snap fit thereon to be locked onto said housing and form a leak tight seal, wherein said end cap includes at least one liquid reservoir for holding reagents and/or liquid sample, said at least one liquid reservoir including pierceable sealing means, and wherein said reagent and sample inlets pierce said pierceable sealing means when said end cap is slid onto said housing;

i) a pattern of analyte-specific receptors bound to an inner surface of the reaction chamber, the patterned area being substantially surrounded by an array of radially oriented microfluidic channels configured to direct fluid from said reaction chamber radially outward to said first flow passageway; and

j) said optical element directs a light beam to the reaction chamber for interrogating the pattern of analyte specific receptors, wherein the light beam interacts with the pre-selected pattern of analyte specific receptors and analytes bound thereto, and wherein the light beam that interacts with the pre-selected pattern of analyte-specific receptors and analytes bound thereto is a diffracted light beam which is directed away from said inner surface by said optical element.

2. The vessel according to claim 1 wherein said reagent and sample inlets are spaced apart, and wherein said second flow passageway includes branches each associated with a sample or reagent inlet, said branches converging to a common passageway which terminates at said reaction chamber.

3. The vessel according to claim 1 wherein said end cap includes a label affixed thereto to indicate identity of the reagents and liquid sample sealed therein.

4. The vessel according to claim 1 including fluid displacement means associated with each of the liquid reservoirs for displacing the reagents and sample into said second flow passageway.

5. The vessel according to claim 4 wherein said fluid displacement means includes means for pressurizing each of said liquid reservoirs to force liquid contents contained therein into said second flow passageway.

6. The vessel according to claim 4 wherein said fluid displacement means includes a piston assembly with a piston associated with each liquid reservoir and means for moving each piston independent of the other pistons.

7. The vessel according to claim 1 wherein said second flow passageway has dimensions selected to give capillary flow therein so that upon introduction of the reagents and sample to their respective inlets, capillary forces draw them into the second flow passageway towards said reaction chamber.

8. The vessel according to claim 1 wherein said first flow passageway has dimensions selected to give capillary flow therein so that the reagents and sample are drawn out of said reaction chamber after a designated diffraction assay by capillary forces to draw them into the waste chamber.

9. The vessel according to claim 1 wherein at least one of said first and second housing sections, or both, includes a keying mechanism, said keying mechanism providing a mechanical interface and alignment to an analyser instrument which is used in conjunction with said vessel.

10. The vessel according to claim 9 wherein said keying mechanism includes at least one guide rail extending along an outer surface of at least one of the first and second housing sections the first and second housing sections.

11. The vessel according to claim 1 wherein one of said first and second housing sections, or both, includes a label affixed thereto.

12. The vessel according to claim 11 wherein said label is one of a human readable label and a machine readable label, or both.

13. The vessel according to claim 12 wherein said machine readable label is selected from the group consisting of bar codes and radio frequency (RF) identifiers.

14. The vessel according to claim 1 including fluorescent compounds bound to, or interacting with, said pattern of analyte-specific receptors which can be induced by an incident beam of light, which may or may not be coherent, to fluoresce and reemit light at a shifted wavelength in a more or less non-directional manner, which is detectable by a photodetector.

15. The vessel according to claim 1 including chemiluminescent compounds bound to, or interacting with, said pattern of analyte-specific receptors which can be induced by an incident beam of light, which may or may not be coherent, to chemiluminesce and emit light in a more or less non-directional manner, which is detectable by a photodetector.

16. A disposable reaction vessel comprising:

a) a housing including a first housing section and a second housing section form fitted to said first housing section so that when the first and second housing sections are assembled to form said housing they are in sealing relationship;

b) a reaction chamber located inside of said housing;

c) an optical element integrally formed on an outside surface of the second housing section and located adjacent to said reaction chamber on the inside of the housing, said second housing section being at least partially constructed of a material which will transmit light to and from the reaction chamber;

d) a waste reservoir located on an interior of said housing;

e) a first flow passageway between said reaction chamber and said waste reservoir;

f) at least one sample inlet and at least one reagent inlet located at a first end portion of said housing;

g) a second flow passageway between said reagent and sample inlets and said reaction chamber;

h) an end cap having a size and shape to slide over said first end portion and snap fit thereon to be locked onto said housing and form a leak tight seal, and wherein said end cap includes a separate liquid reservoir for holding reagents or liquid sample associated with each of the inlets and pierceable sealing means sealing each liquid reservoir, wherein each inlet includes a piercing means associated therewith for piercing said pierceable sealing means and at least one of said liquid reservoirs being pre-filled with selected reagents;

i) a pattern of analyte-specific receptors bound to an inner surface of the reaction chamber, the patterned area being substantially surrounded by an array of radially oriented microfluidic channels configured to direct fluid from said reaction chamber radially outward to said first flow passageway; and

j) said optical element to direct a light beam to the reaction chamber for interrogating the pattern of analyte specific receptors, wherein the light beam interacts with the pre-selected pattern of analyte specific receptors and analytes bound thereto, and wherein the light beam that interacts with the pre-selected pattern of analyte-specific receptors and analytes bound thereto is a diffracted light beam which is directed away from said inner surface by said optical element.

17. The vessel according to claim 16 including fluid displacement means associated with each of the liquid reservoirs for displacing the reagents and sample into said second flow passageway.

18. The vessel according to claim 17 wherein said fluid displacement means includes means for pressurizing each of said liquid reservoirs to force liquid contents contained therein into said second flow passageway.

19. The vessel according to claim 17 wherein said fluid displacement means includes a piston assembly with a piston associated with each liquid reservoir and means for moving each piston independent of the other pistons.

20. The vessel according to claim 16 wherein said second flow passageway has dimensions selected to give capillary flow therein so that upon introduction of the reagents and sample to their respective inlets, capillary forces draw them into the second flow passageway towards said reaction chamber.

21. The vessel according to claim 16 wherein said first flow passageway has dimensions selected to give capillary flow therein so that the reagents and sample are drawn out of said reaction chamber after a designated diffraction assay by capillary forces to draw them into the waste chamber.

22. The vessel according to claim 16 wherein at least one of said first and second housing sections, or both, includes a keying mechanism, said keying mechanism providing a mechanical interface and alignment to an analyser instrument which is used in conjunction with said vessel.

23. The vessel according to claim 22 wherein said keying mechanism includes at least one guide rail extending along an outer surface of at least one of the first and second housing sections.

24. The vessel according to claim 16 wherein said end cap includes a label affixed thereto to indicate identity of the reagents and liquid sample sealed therein.

25. The vessel according to claim 16 wherein one of said first and second housing sections, or both, includes a label affixed thereto.

26. The vessel according to claim 25 wherein said label is one of a human readable label and a machine readable label, or both.

27. The vessel according to claim 26 wherein said machine readable label is selected from the group consisting of bar codes and radio frequency (RF) identifiers.

28. The vessel according to claim 16 including fluorescent compounds bound to, or interacting with, said pattern of analyte-specific receptors which can be induced by an incident beam of light, which may or may not be coherent, to fluoresce and reemit light at a shifted wavelength in a more or less non-directional manner, which is detectable by a photodetector.

29. The vessel according to claim 16 including chemiluminescent compounds bound to, or interacting with, said pattern of analyte-specific receptors which can be induced by an incident beam of light, which may or may not be coherent, to chemiluminesce and emit light in a more or less non-directional manner, which is detectable by a photodetector.