Method of obtaining a product sugar stream from cellulosic biomass
First Claim
1. A process for obtaining a product sugar stream from cellulosic biomass comprising cellulose and hemicellulose, the process comprising:
- a) pretreating the cellulosic biomass at a pH of about 0.4 to about 2.0 by adding one or more than one acid to the cellulosic biomass to hydrolyze a portion of the cellulose and at least a portion of the hemicellulose in the cellulosic biomass to produce a pretreated cellulosic biomass comprising glucose, acetic acid and a sugar monomer selected from the group consisting of xylose, arabinose, mannose, galactose and a combination thereof;
b) adding one or more than one base to the pretreated cellulosic biomass to adjust the pretreated cellulosic biomass to a pH of about 4.0 to about 6.0, thereby producing a neutralized cellulosic biomass comprising inorganic salt and acetate salt;
c) hydrolyzing the neutralized cellulosic biomass with cellulase enzymes to produce a crude sugar stream;
d) separating insoluble residue from the crude sugar stream to produce a clarified sugar stream;
e) treating the clarified sugar stream by ion exclusion chromatography with a cation exchange resin at a pH from 5.0 to 10.0 to produce one or more than one raffinate stream comprising the inorganic salt and acetate salt and a product sugar stream comprising sugar, wherein the concentration of acetic acid plus acetate salt in the clarified sugar stream fed to the ion exclusion chromatography step is greater than 5 g/L; and
f) recovering the product sugar stream.
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Abstract
The invention relates to a process for obtaining a product sugar stream from a cellulosic biomass, comprising pretreating the cellulosic biomass to hydrolyze a portion of the cellulose and hemicellulose to produce glucose, acetic acid and a sugar monomer, adding base to the pretreated cellulosic biomass to produce inorganic salt and acetate salt; hydrolyzing the neutralized cellulosic biomass with cellulase enzymes and separating insoluble residue from the resulting sugar stream; treating the clarified sugar stream using exclusion chromatography at a pH of 5 to 10 to produce a product sugar stream.
42 Citations
48 Claims
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1. A process for obtaining a product sugar stream from cellulosic biomass comprising cellulose and hemicellulose, the process comprising:
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a) pretreating the cellulosic biomass at a pH of about 0.4 to about 2.0 by adding one or more than one acid to the cellulosic biomass to hydrolyze a portion of the cellulose and at least a portion of the hemicellulose in the cellulosic biomass to produce a pretreated cellulosic biomass comprising glucose, acetic acid and a sugar monomer selected from the group consisting of xylose, arabinose, mannose, galactose and a combination thereof; b) adding one or more than one base to the pretreated cellulosic biomass to adjust the pretreated cellulosic biomass to a pH of about 4.0 to about 6.0, thereby producing a neutralized cellulosic biomass comprising inorganic salt and acetate salt; c) hydrolyzing the neutralized cellulosic biomass with cellulase enzymes to produce a crude sugar stream; d) separating insoluble residue from the crude sugar stream to produce a clarified sugar stream; e) treating the clarified sugar stream by ion exclusion chromatography with a cation exchange resin at a pH from 5.0 to 10.0 to produce one or more than one raffinate stream comprising the inorganic salt and acetate salt and a product sugar stream comprising sugar, wherein the concentration of acetic acid plus acetate salt in the clarified sugar stream fed to the ion exclusion chromatography step is greater than 5 g/L; and f) recovering the product sugar stream. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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22. A process for producing ethanol comprising:
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a) obtaining cellulosic biomass comprising cellulose and hemicellulose from a feedstock selected from the group consisting of an agricultural waste, corn stover, wheat straw, barley straw, canola straw, oat straw, rice straw, soybean stover, a grass, switch grass, miscanthus, cord grass, reed canary grass, a forestry residue, aspen wood or sawdust, a sugar residue, bagasse and beet pulp; b) pretreating the cellulosic biomass at a pH of about 0.4 to about 2.0 by adding one or more than one acid to the cellulosic biomass to hydrolyze a portion of the cellulose and at least a portion of the hemicellulose in the cellulosic biomass to produce a pretreated cellulosic biomass comprising glucose, acetic acid and a sugar monomer selected from the group consisting of xylose, arabinose, mannose, galactose and a combination thereof; c) adding one or more than one base to the pretreated cellulosic biomass to adjust the pretreated cellulosic biomass to a pH of about 4.0 to about 6.0, thereby producing a neutralized cellulosic biomass comprising inorganic salt and acetate salt; d) hydrolyzing the neutralized cellulosic biomass with cellulase enzymes to produce a crude sugar stream; e) separating insoluble residue from the crude sugar stream to produce a clarified sugar stream; f) treating the clarified sugar stream by ion exclusion chromatography with a cation exchange resin at a pH from 5.0 to 10.0 to produce one or more than one raffinate stream comprising the inorganic salt and acetate salt and a product sugar stream comprising sugar, wherein the concentration of acetic acid plus acetate salt in the clarified sugar stream fed to the ion exclusion chromatography step is greater than 5 g/L; g) recovering the product sugar stream, and the one or more than one raffinate stream; and h) fermenting the sugar in the product sugar stream to ethanol. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40)
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41. A process for obtaining a product sugar stream from a crude sugar stream, the crude sugar stream produced from conversion of cellulosic biomass to sugar, the process comprising:
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a) separating insoluble residue from the crude sugar stream to produce a clarified sugar stream; b) treating the clarified sugar stream by ion exclusion chromatography with a cation exchange resin at a pH from 5.0 to 10.0 to produce one or more than one raffinate stream comprising sulfate and acetate salts, and a product stream comprising sugar, wherein the concentration of acetic acid plus acetate salt in the clarified sugar stream fed to the ion exclusion chromatography step is greater than 5 g/L; and c) obtaining the product sugar stream. - View Dependent Claims (42, 43, 44)
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45. A process for obtaining one or more than one raffinate stream enriched in inorganic salt and acetate salt, the process comprising:
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a) obtaining a clarified stream comprising sugar, inorganic salt and at least one of an acetate salt and acetic acid originating from a previous hydrolysis of the cellulosic biomass; and b) treating the clarified stream by ion exclusion chromatography with a cation exchange resin at a pH from 5.0 to 10.0 to produce the one or more than one raffinate stream enriched in inorganic salt and acetate salt, and a stream comprising sugar, wherein the concentration of acetic acid plus acetate salt in the clarified stream fed to the ion exclusion chromatography step is greater than 5 g/L. - View Dependent Claims (46, 47, 48)
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Specification