Nitrite detection technique

• US 8,003,399 B2
• Filed: 08/31/2005
• Issued: 08/23/2011
• Est. Priority Date: 08/31/2005
• Status: Expired due to Fees

• Current Assignee: Kimberly-Clark Worldwide Incorporated (Kimberly-Clark Corporation)
• Original Assignee: Kimberly-Clark Worldwide Incorporated (Kimberly-Clark Corporation)
• Inventors: Song, Xuedong, Kaylor, Rosann Marie Matthews
• Primary Examiner(s): Menon; Krishnan S
• Assistant Examiner(s): FRITCHMAN, REBECCA M
• Application Number:
  US11/217,099
• Publication Number:
  US 20070048182A1
• Time in Patent Office: 2,183 Days
• Field of Search: 436/110, 436/514, 436/518, 422/56, 422/61, 435/5, 435/7.92
• US Class Current: 436/110
• CPC Class Codes: G01N 2800/348 Urinary tract infections
• CPC Class Codes: G01N 33/526 the element being adapted for a specific analyte
• CPC Class Codes: Y10T 436/173076 Nitrite or nitrate

Abstract

A diagnostic test kit for detecting the presence or absence of nitrites within a test sample is provided. The test kit comprises an aromatic primary amine that is capable of reacting with a nitrite to form a diazonium ion. The test kit also comprises a lateral flow device that comprises a chromatographic medium and an absorbent material that receives the test sample after flowing through the chromatographic medium. The chromatographic medium defines a detection zone within which is contained a detection reagent (e.g., nucleophilic aromatic amine) that is capable of reacting with the diazonium ion to form an indicator (e.g., azo compound). The indicator exhibits a color that is different than the color of the detection reagent.

Claims

1. A diagnostic test kit for detecting the presence or absence of nitrites within a urine test sample, the test kit comprising:

a reagent pad on which an aromatic primary amine is disposed that is capable of reacting with a nitrite or nitrite derivative in the urine test sample to form a diazonium ion; and

a lateral flow device that is in fluid communication with the reagent pad and comprises;

a porous membrane defining a discrete detection zone that is separate and distinct from the reagent pad and within which is contained a nucleophilic aromatic amine detection reagent, the detection reagent being capable of reacting with the diazonium ion to form an azo indicator, the azo indicator exhibiting a color that is different than the color of the detection reagent; and

an absorbent material that receives the urine test sample after flowing through the porous membrane.

2. The diagnostic test kit of claim 1, wherein the aromatic primary amine is p-arsalinic acid, sulfanilamide, or a derivative thereof.

3. The diagnostic test kit of claim 1, wherein the detection reagent comprises N-1-naphthylethylene diamine or a derivative thereof.

4. The diagnostic test kit of claim 1, wherein the detection reagent comprises a macromolecular moiety and an aromatic amine moiety.

5. The diagnostic test kit of claim 4, wherein the macromolecular moiety is formed from a polymer.

6. The diagnostic test kit of claim 5, wherein the polymer is a polysaccharide.

7. The diagnostic test kit of claim 4, wherein the macromolecular moiety is formed from a particle.

8. The diagnostic test kit of claim 4, wherein the aromatic amine moiety is formed from N-1-naphthylethylene diamine or a derivative thereof.

9. The diagnostic test kit of claim 1, wherein the aromatic primary amine is disposed on the lateral flow assay device.

10. The diagnostic test kit of claim 1, wherein the aromatic primary amine comprises aniline;

4-chloroaniline;

4-bromoaniline;

2,4,6-tribromoaniline;

2,4,6-trichloroaniline;

α

-trifluoro-m-toluidene;

ortho-toluidine;

m- and p-aminophenol;

ortho-tolidine;

sulfanilamide, p-aminobenzoic acid;

1-amino-8-hydroxynaphthalene-3 , 6-disulphonic acid;

aminoacetoanilide;

aminophenyl ether, p-arsalinic acid;

4-amino-1-naphthalenecarbonitrile, or derivatives thereof.

11. The diagnostic test kit of claim 1, wherein the detection reagent comprises 8-hydroxyjulolidine, N,N-dimethylaniline;

methylenedianiline;

benzidine;

benzoquinoline;

aminoquinoline;

m-phenylenediamine;

α

-trifluoro-m-toluidene;

ortho-toluidine;

m-aminophenol;

ortho-tolidine;

N-1-naphthylethylene diamine, or a derivative thereof.

12. The diagnostic test kit of claim 7, wherein the particle is a carboxylated latex particle.

13. The diagnostic test kit of claim 1, wherein the indicator is an aromatic azo compound.

14. The diagnostic test kit of claim 1, wherein the lateral flow device further comprises a sample pad configured to receive the test sample.

15. The diagnostic test kit of claim 14, wherein the sample pad also defines a quenching zone that contains an agent capable of reacting with a contaminant in the test sample.

16. The diagnostic test kit of claim 1, wherein the absorbent material includes nitrocellulose, a cellulosic material, a porous polyethylene pad, glass fiber filter paper, or a combination thereof.

17. The diagnostic test kit of claim 1, wherein the nitrite derivative is nitrous acid.

18. The diagnostic test kit of claim 1, wherein the detection zone is located laterally downstream from the reagent zone.