Calibrating dispensing device performance for complex and/or non-aqueous liquids
First Claim
1. A method to create a test-stock mixture to be used in the determination of a volume of the test-stock mixture to be dispensed from a liquid handling device to be calibrated, the test-stock mixture including a test liquid of interest, the method comprising the step of mixing the test liquid with a stock solution, which stock solution includes a first dye with a known absorbance characteristic to form the test-stock mixture, wherein a ratio of test liquid to stock solution is selected to establish for the test-stock mixture flow characteristics that correspond to flow characteristics of the test liquid before mixing with the stock solution while maintaining in the test-stock mixture a distinction between the known absorbance characteristic of the first dye in the stock solution and a known absorbance characteristic of a second dye.
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Abstract
A method for determining the volume of a test liquid aliquot used to calibrate a liquid delivery device. The method is useful for determining the volume of an aliquot of a test liquid which is complex, non-aqueous or both. A mixture contains the test liquid and a stock solution including a first dye, the first dye having absorbance characteristics which are measurably distinguishable from the absorbance characteristics of a second dye. The mixture is mixed with a diluent including the second dye to form a sample solution used to measure absorbances of the first dye and the second dye. The test liquid and the stock solution are combined gravimetrically in a controlled ratio so that the flow characteristics of the test-stock mixture correspond to the flow characteristics of the test liquid and without affecting the ability to distinguish the absorbance characteristics between the first dye and the second dye.
37 Citations
12 Claims
- 1. A method to create a test-stock mixture to be used in the determination of a volume of the test-stock mixture to be dispensed from a liquid handling device to be calibrated, the test-stock mixture including a test liquid of interest, the method comprising the step of mixing the test liquid with a stock solution, which stock solution includes a first dye with a known absorbance characteristic to form the test-stock mixture, wherein a ratio of test liquid to stock solution is selected to establish for the test-stock mixture flow characteristics that correspond to flow characteristics of the test liquid before mixing with the stock solution while maintaining in the test-stock mixture a distinction between the known absorbance characteristic of the first dye in the stock solution and a known absorbance characteristic of a second dye.
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8. A method for determining the volume of a liquid aliquot to be dispensed from a handling device, comprising the steps of:
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a. making a test-stock mixture by mixing a test liquid having a known weight and a known density value with a stock solution having a known weight and a known density value, the stock solution including a first dye with a known absorbance characteristic; b. adding an aliquot of the test-stock mixture to a liquid holder; c. adding a diluent to the test-stock mixture in the liquid holder to produce a sample solution, the diluent including only a second dye having a known absorbance characteristic different from the known absorbance characteristic of the first dye, wherein a ratio of the known weight of the test liquid to the known weight of the stock solution is selected to establish for the test-stock mixture flow characteristics that correspond to flow characteristics of the test liquid before mixing with the stock solution while maintaining in the test-stock mixture the difference in known absorbance characteristics between the first dye and the second dye; d. measuring the absorbance values of the first dye and the second dye in the sample solution at a first wavelength and a second wavelength; and e. determining the volume of the test-stock mixture aliquot by using the measured absorbance values of the sample solution and the absorbance per pathlength of either or both of the first dye and second dye. - View Dependent Claims (9, 10, 11, 12)
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Specification