Method of determining a condition of a tissue
First Claim
1. A method for determining a condition of a tissue, the method comprising:
- illuminating a tissue with light at an excitation wavelength;
detecting a first and second intensity of received electromagnetic radiation from the tissue upon illumination at said excitation wavelength, wherein the first intensity corresponds to a first wavelength and the second intensity corresponds to a second wavelength different from the first wavelength;
normalizing the first and second intensities with an intensity corresponding to an isosbestic point; and
determining a condition of the tissue based at least in part on the normalized first and second intensities.
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Abstract
A system and method for the in situ discrimination of healthy and diseased tissue. A fiberoptic based probe is employed to direct ultraviolet illumination onto a tissue specimen and to collect the fluorescent response radiation. The response radiation is observed at three selected wavelengths, one of which corresponds to an isosbestic point. In one example, the isosbestic point occurs at about 431 nm. The intensities of the observed signals are normalized using the 431 nm intensity. A score is determined using the ratios in a discriminant analysis. The tissue under examination is resected or not, based on the diagnosis of disease or health, according to the outcome of the discriminant analysis.
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Citations
16 Claims
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1. A method for determining a condition of a tissue, the method comprising:
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illuminating a tissue with light at an excitation wavelength; detecting a first and second intensity of received electromagnetic radiation from the tissue upon illumination at said excitation wavelength, wherein the first intensity corresponds to a first wavelength and the second intensity corresponds to a second wavelength different from the first wavelength; normalizing the first and second intensities with an intensity corresponding to an isosbestic point; and determining a condition of the tissue based at least in part on the normalized first and second intensities. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method for determining a condition of a tissue, the method comprising:
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illuminating a tissue with light at an excitation wavelength; detecting a first and second intensity of received electromagnetic radiation from the tissue upon illumination at said excitation wavelength, wherein the first intensity corresponds to a first wavelength and the second intensity corresponds to a second wavelength different from the first wavelength; normalizing the first and second intensities with an intensity corresponding to an isosbestic point to produce a normalized first intensity and a normalized second intensity, wherein the isosbestic point is a wavelength in a band at 431 nm with a bandwidth under 5 nm; and determining a condition of the tissue based at least in part on the normalized first and second intensities.
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Specification