Apparatus for elucidating reaction dynamics of photoreactive compounds from optical signals affected by an external magnetic field

• US 8,005,528 B2
• Filed: 03/05/2009
• Issued: 08/23/2011
• Est. Priority Date: 09/26/2003
• Status: Active Grant

• Current Assignee: Institut national d'optique
• Original Assignee: Institut national d'optique
• Inventors: Long, William F.
• Primary Examiner(s): Casler; Brian
• Assistant Examiner(s): KISH, JAMES M
• Application Number:
  US12/398,593
• Publication Number:
  US 20090198114A1
• Time in Patent Office: 901 Days
• Field of Search: 600/407, 600/473, 600/475, 600476-478
• US Class Current: 600/407
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: A61B 5/14551 for measuring blood gases
• CPC Class Codes: A61N 2/002 in combination with another treatment
• CPC Class Codes: A61N 5/062 Photodynamic therapy, i.e. excitation of an agent

Abstract

A device elucidates reaction dynamics of photoreactive compounds present in a region of interest (ROI) of a tissue, the ROI being submitted to a controllably-varying and spatially homogenous magnetic field. The device includes at least one electrical coil or magnet for externally applying a magnetic field to the ROI. The device further includes light sources for probing the ROI with a probe optical beam and for illuminating the ROI with an optical beam having a wavelength and power level suitable for activating the photoreactive compounds. Collectors are also provided to collect the light emitted from various photoluminescence mechanisms that occur in the optically-activated ROI when the probe beam is incident on it and then to detect the collected light over predetermined wavelength bands. Finally, a computer is used to analyze the detected light for assessing the effects of the external magnetic field applied to the ROI.

Claims

1. An apparatus for elucidating reaction dynamics of photoreactive compounds present in a region of interest (ROI) of a tissue submitted to a substantially homogeneous magnetic field, said apparatus comprising:

means for applying said substantially homogeneous magnetic field to said ROI, said means being external to said ROI and not in contact with said ROI;

means for controllably varying the strength of said magnetic field according to a predetermined temporal pattern;

a Hall-effect probe connected to a magnetic flux meter to measure the strength of said magnetic field;

means for illuminating said ROI with a therapeutic optical beam, the combination of applying a magnetic field and illuminating said ROI with said therapeutic optical beam activating chemical reactions in said photoreactive compounds and affecting the subsequent evolution of said chemical reactions;

means for illuminating said ROI with a probe optical beam;

means for collecting light emitted from said ROI, to produce a collected light, said emitted light consisting of the portion of said probe optical beam that is transmitted through said ROI or the portion of said probe optical beam that is backscattered from said ROI according to a photoluminescence process;

means for detecting said collected light over at least one predetermined wavelength band; and

means for analyzing said detected light.

2. The apparatus according to claim 1, further including a pulse oximeter, an oxygen electrode, or both.

3. The apparatus according to claim 1, wherein said means for applying a magnetic field include at least one permanent magnet.

4. The apparatus according to claim 3, wherein said means for controllably varying the strength and temporal pattern of said magnetic field include means for moving said at least one permanent magnet.

5. The apparatus according to claim 4, wherein said temporal pattern of said magnetic field is selected from the group consisting of a square-wave modulation, a sinusoidal modulation, a constant value, a predetermined non periodic function of limited duration, or of any combination thereof.

6. The apparatus according to claim 1, wherein said means for analyzing said detected light are adapted to generate processed data, including luminescence lifetime calculations, difference and ratiometric processing, and correlating between at least two variables.

7. The apparatus according to claim 6, wherein said processed data is outputted visually as a map or a graph.

8. The apparatus according to claim 1, wherein said means for applying a magnetic field include at least one electrical coil.

9. The apparatus according to claim 8, wherein said means for controllably varying the strength and temporal pattern of said magnetic field include an electrical current source connected to said at least one electrical coil.

10. The apparatus according to claim 9, wherein said temporal pattern of said magnetic field is selected from the group consisting of a square-wave modulation, a sinusoidal modulation, a constant value, a predetermined non periodic function of limited duration, or of any combination thereof.