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Enzymatic electrochemical biosensor

  • US 8,007,656 B2
  • Filed: 10/22/2004
  • Issued: 08/30/2011
  • Est. Priority Date: 10/24/2003
  • Status: Active Grant
First Claim
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1. An electrochemical sensor strip, comprising:

  • a base;

    a first electrode on the base;

    a first reagent layer on the first electrode, the first reagent layer comprising an electroactive organic molecule and an oxidoreductase capable of facilitating a redox reaction of an analyte;

    a second electrode on the base; and

    a second reagent layer on the second electrode, the second reagent layer consisting essentially of a redox pair comprising a first soluble redox species and a second soluble redox species, the redox pair selected from the group consisting of an organotransition metal complex, a transition metal coordination complex, and mixtures thereof, wherethe first soluble redox species is capable of undergoing a redox reaction opposite that of the analyte, and wherethe molar ratio of the first soluble redox species to the second soluble redox species is greater than 1.2;

    1 prior to use of the sensor strip in an analysis.

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