Real-time detection of influenza virus
First Claim
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1. A method of detecting an influenza viral particle in a bodily fluid from a subject, comprising:
- a) allowing a sample of said bodily fluid suspected to contain said influenza viral particle to react with a first immunoassay reagent and a second immunoassay reagent, both of which being contained within an immunoassay assembly of a cartridge, wherein said first immunoassay reagent binds to a hemagglutinin molecule to form a first immune complex on said influenza viral particle, and wherein said second immunoassay reagent binds to a neuraminidase molecule to form a second immune complex on said influenza viral particle, said immune complexes yielding one or more detectable signals that indicate the simultaneous presence of hemagglutinin and neuraminidase on said influenza viral particle wherein either the first or second immunoassay reagent is immobilized on a solid support; and
b) detecting said one or more detectable signals generated from said immune complexes, wherein said simultaneous presence of hemagglutinin and neuraminidase is indicative of the presence of said influenza viral particle.
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Abstract
The present invention provides system and methods for detecting an analyte indicative of an influenza viral infection in a sample of bodily fluid. The present invention also provides for systems and method for detection a plurality of analytes, at least two of which are indicative of an influenza viral infection in a sample of bodily fluid.
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22 Claims
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1. A method of detecting an influenza viral particle in a bodily fluid from a subject, comprising:
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a) allowing a sample of said bodily fluid suspected to contain said influenza viral particle to react with a first immunoassay reagent and a second immunoassay reagent, both of which being contained within an immunoassay assembly of a cartridge, wherein said first immunoassay reagent binds to a hemagglutinin molecule to form a first immune complex on said influenza viral particle, and wherein said second immunoassay reagent binds to a neuraminidase molecule to form a second immune complex on said influenza viral particle, said immune complexes yielding one or more detectable signals that indicate the simultaneous presence of hemagglutinin and neuraminidase on said influenza viral particle wherein either the first or second immunoassay reagent is immobilized on a solid support; and b) detecting said one or more detectable signals generated from said immune complexes, wherein said simultaneous presence of hemagglutinin and neuraminidase is indicative of the presence of said influenza viral particle. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 22)
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18. A method of detecting an influenza A viral particle in a sample of bodily fluid suspected to contain the particle, comprising:
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a) providing an immunoassay assembly, wherein the assembly comprises a first antibody that binds specifically to a first antigen, wherein the first antibody is bound to a surface of the assembly; b) reacting the sample of bodily fluid in the immunoassay assembly in the presence of immunoassay reagents comprising a second antibody that binds specifically to a second antigen which is distinct from the first antigen, wherein the second antibody is labeled with a detectable label, wherein either the first antigen comprises a hemagglutinin and the second antigen comprises a neuraminidase, or the first antigen comprises a neuraminidase and the second antigen comprises a hemagglutinin, and wherein said reaction yields an immune complex comprising the first antibody bound to the first antigen, and the second antibody bound to the second antigen; c) washing the assembly to remove excess immunoassay reagents; and d) detecting a signal generated by the detectable label from the immune complex, thereby indicating the presence of the particle. - View Dependent Claims (19, 20, 21)
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Specification