Genetic variation in pro-melanin-concentrating hormone gene affects carcass traits in cattle
First Claim
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1. A method of identifying a Bos sp, animal likely to have a phenotype of higher grade fat depth and higher average fat depth, lower shear force and improved palatability, comprising:
- (a) isolating a nucleic acid sample from the animal, and(b) determining, by direct detection of the nucleotides present at position 38 in SEQ ID NO;
1, whether the genotype at position 38 in SEQ ID NO;
1, in the nucleic acid sample, is “
AA”
, “
TT”
or “
AT”
,(c) identifying an animal with the “
AA”
or “
AT”
genotype as likely to have the phenotype or identifying an animal with the “
TT”
genotype as not likely to have the phenotype.
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Abstract
An A-to-T single nucleotide polymorphism (SNP) identified at position −134 relative to the ATG start codon, in the Pro-Melanin-Concentrating Hormone (PMCH) gene of Bos taurus and Bos indicus animals of both British and Continental type, is associated with changes in the average fat and grade fat. The A allele occurred in 67% of cattle examined and was associated with higher average fat and grade fat levels. The cattle industry may make use of these findings to genetically select for, and/or sort, cattle using this SNP.
18 Citations
16 Claims
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1. A method of identifying a Bos sp, animal likely to have a phenotype of higher grade fat depth and higher average fat depth, lower shear force and improved palatability, comprising:
-
(a) isolating a nucleic acid sample from the animal, and (b) determining, by direct detection of the nucleotides present at position 38 in SEQ ID NO;
1, whether the genotype at position 38 in SEQ ID NO;
1, in the nucleic acid sample, is “
AA”
, “
TT”
or “
AT”
,(c) identifying an animal with the “
AA”
or “
AT”
genotype as likely to have the phenotype or identifying an animal with the “
TT”
genotype as not likely to have the phenotype. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A method of regulating the time to finishing, in a group of Bos sp, animals entering a feedlot, said method comprising:
-
(a) determining, by direct detection of the nucleotides present at position 38 in SEQ ID NO;
1, whether the animals in the group have an “
AA”
, “
AT”
or “
TT”
genotype at position 38 in SEQ ID NO;
1,(b) providing each animal in the group a diet that is selected according to its genotype at position 38 in SEQ ID NO;
1, and(c) feeding animals of the same genotype the same diet. - View Dependent Claims (10, 11)
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12. A method of improving the quality and consistency of the average fat depth and grade fat depth of carcasses obtained from a group of Bos sp, animals from a feedlot, or the quality and consistency of the shear force and palatability of beef obtained from these animals, said method comprising:
-
(a) genotyping the animals in the group, by direct detection of the nucleotides present at position 38 in SEQ ID NO;
1, to determine whether they have an “
AA”
, “
AT”
or “
TT”
genotype at position 38 in SEQ ID NO;
1,(b) sorting the animals into sub-groups according to their genotype at position 38 in SEQ ID NO;
1, and(c) feeding the animals in any one sub-group the same diet for the same length of time. - View Dependent Claims (13)
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14. A method of breeding Bos sp, animals, based on the knowledge of the animals'"'"' genotype, comprising the steps of:
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(a) obtaining a nucleic acid sample from the animals; (b) analysing the nucleic acid sample to determine by direct detection of the nucleotides present at position 38 in SEQ ID NO;
1, the genotype at position 38 in SEQ ID NO;
1 in the animals, wherein the genotype will be one of “
AA”
, “
AT”
, or “
TT”
at this position,(c) selecting a first animal and a second animal for breeding, on the basis of their genotype at this position, and (d) breeding the first animal with the second animal. - View Dependent Claims (15)
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16. A method of increasing the grade fat depth and average fat depth, lowering the shear force and improving palatability, in a group of Bos sp, animals, comprising:
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(a) obtaining a nucleic acid sample from the animals; (b) analysing the nucleic acid sample to determine a genetic predisposition of Bos sp, animals to have higher average fat depth and higher grade fat depth, lower shear force and better palatability, by determining, by direct detection of the nucleotides present at position 38 in SEQ ID NO;
1, their genotype at position 38 in SEQ ID NO;
1,(c) selecting animals that have the “
A”
allele at position 38 in SEQ ID NO;
1 for inclusion in the group, and(d) removing animals that have only the “
T”
allele from the group.
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Specification