Calibration procedure for investigating biological systems

• US 8,008,064 B2
• Filed: 02/15/2007
• Issued: 08/30/2011
• Est. Priority Date: 03/11/2002
• Status: Expired due to Fees

• Current Assignee: Janusz B. Pawliszyn
• Original Assignee: Janusz B. Pawliszyn
• Inventors: Pawliszyn, Janusz B.
• Primary Examiner(s): LAM, ANN Y
• Application Number:
  US11/706,167
• Publication Number:
  US 20070148782A1
• Time in Patent Office: 1,657 Days
• Field of Search: None
• US Class Current: 435/287.1
• CPC Class Codes: A61B 10/0045 Devices for taking samples of body liquids devices for taking blood samples A61B5/15
• CPC Class Codes: A61B 17/3403 Needle locating or guiding means guides for suture needles A61B17/0482; guiding or tracking by nuclear magnetic resonance G01R33/285
• CPC Class Codes: A61B 5/14514 using means for aiding extraction of interstitial fluid, e.g. microneedles or suction
• CPC Class Codes: A61B 5/15 Devices for taking samples of blood hypodermic syringes A61M5/178
• CPC Class Codes: A61B 5/150022 for capillary blood or interstitial fluid
• CPC Class Codes: A61B 5/15003 for venous or arterial blood
• CPC Class Codes: A61B 5/150099 by negative pressure, other than vacuum extraction into a syringe by pulling on the piston rod or into pre-evacuated tubes
• CPC Class Codes: A61B 5/150229 Pumps for assisting the blood sampling
• CPC Class Codes: A61B 5/150389 Hollow piercing elements, e.g. canulas, needles, for piercing the skin
• CPC Class Codes: A61B 5/150503 Single-ended needles hollow piercing element tip design A61B5/150396, needle sets for catheters A61M25/06, needles for bringing media into the body A61M5/32
• CPC Class Codes: A61B 5/151 Devices specially adapted for taking samples of capillary blood, e.g. by lancets , needles or blades
• CPC Class Codes: A61B 5/153 Devices specially adapted for taking samples of venous or arterial blood, e.g. with syringes
• CPC Class Codes: A61B 5/157 Devices characterised by integrated means for measuring characteristics of blood
• CPC Class Codes: A61B 5/417 the bone marrow
• CPC Class Codes: B01L 2300/046 Function or devices integrated in the closure
• CPC Class Codes: B01L 2300/069 Absorbents; Gels to retain a fluid
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0838 Capillaries
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: G01N 1/405 by adsorption or absorption
• CPC Class Codes: G01N 2001/4016 : being a selective membrane, e.g. dialysis or osmosis
• CPC Class Codes: G01N 2030/009 : Extraction
• CPC Class Codes: G01N 2030/062 : extracting sample from raw material
• CPC Class Codes: G01N 2035/1053 : for separating part of the liquid, e.g. filters, extraction phase
• CPC Class Codes: G01N 35/028 : having reaction cells in the form of microtitration plates
• CPC Class Codes: H01J 49/0431 : for liquid samples interfaces to liquid chromatographs G01N30/7233
• CPC Class Codes: Y10S 436/807 : Apparatus included in process claim, e.g. physical support structures
• CPC Class Codes: Y10T 436/105831 : Protein or peptide standard or control [e.g., hemoglobin, etc.]
• CPC Class Codes: Y10T 436/25 : including sample preparation
• CPC Class Codes: Y10T 436/255 : including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

Use of calibrant in extraction phase is described for quantification of components of interest in samples in laboratory application as well as in on-site monitoring. This approach is particularly useful for in-vivo investigation of living systems.

Claims

1. A solid phase microextraction device for sampling and extracting a component contained in a sample, said device comprising a polymeric extraction phase for absorbing or adsorbing the component of interest from the sample and a calibrant non-specifically adsorbed or absorbed in said polymeric extraction phase, said polymeric extraction phase being sized and shaped to fit into an interface of an analytical instrument so said component and calibrant can be desorbed into said instrument.

2. The device of claim 1 wherein the polymeric extraction phase comprises poly(dimethylsiloxane), polyethylene glycol, poly(divinylbenzene), polypyrrole, or octadecylchlorosilane derivatised silica.

3. A solid phase microextraction device for sampling and extracting a component contained in a sample said device comprising a polymeric extraction phase and a calibrant non-specifically adsorbed or absorbed in said polymeric extraction phase, said polymeric extraction phase being sized and shaped to fit into an interface of an analytical instrument so said component and calibrant can be desorbed into said instrument, said polymeric extraction phase being a coating on a fiber located in a sheath, wherein said sheath is a needle, said needle is attached to a syringe, said syringe having a plunger, said fiber is attached to said plunger so said fiber is movable longitudinally within said needle, said plunger being able to successively expose a length of said fiber outside of said needle to said sample and retract said fiber into said needle out of contact with said sample.

4. The device as claimed in claim 3 wherein said interface is an injector of a gas chromatograph.

5. The device as claimed in claim 3 wherein said interface is a well of a multiwell plate containing desorption solvent.

6. The device as claimed in claim 3 wherein said polymeric extraction phase is a coating on a fiber.

7. The device as claimed in claim 6 wherein said fiber is made of metal or fused silica.

8. The device as claimed in claim 3 wherein said sheath has sealing means.

9. The device as claimed in claim 3 wherein said syringe is a part of an autosampler capable to automatically place said fiber in contact with a vial containing said calibrant to load said calibrant onto said fiber, then transferring said fiber to a sample vial for extraction and then delivering said fiber into said interface for desorption of extracted said component and retained said calibrant into said analytical instrument.

10. The device as claimed in claim 3 wherein said calibrant is an isotopically labeled analogue of said component.

11. The device as claimed in claim 3 wherein said polymeric extraction phase is made of biocompatible polymer.

12. The device as claimed in claim 3 wherein said polymeric extraction phase is made of poly(dimethylsiloxane).

13. A solid phase microextraction device for sampling and extracting a component contained in a sample said device comprising a polymeric extraction phase and a calibrant non-specifically adsorbed or absorbed in said polymeric extraction phase, said polymeric extraction phase being sized and shaped to fit into an interface of an analytical instrument so said component and calibrant can be desorbed into said instrument, said polymeric extraction phase being shaped as a thin membrane, wherein said membrane is attached to a handle, said membrane being folded by rolling the membrane around the handle, there being a sheath into which the rolled up membrane can be inserted said sheath being sized and shaped to fit into said interface of said analytical instrument.

14. The device as claimed in claim 13 wherein said calibrant is an isotopically labeled analogue of said component.

15. The device as claimed in claim 13 wherein said polymeric extraction phase is made of biocompatible polymer.

16. The device as claimed in claim 13 wherein said polymeric extraction phase is made of poly(dimethylsiloxane).