Nonhemolytic optical sensor with enhanced reflectance
First Claim
1. A sensor for measuring concentration of an analyte in an erythrocyte-containing biological fluid comprising:
- an optical material having a surface site wherein the surface site of the optical material presents a textured field of projections and micro-wells, the projections being spaced apart for excluding the erythrocytes from entering into the micro-wells; and
a deposit disposed on the surface site of the optical material for contacting the biological fluid, the deposit being disposed in each of the micro-wells and comprising an analyte-reactive reagent and an array of spherical styrene-acrylic beads penetrable to transport of the biological fluid exclusive of the erythrocytes contained therein.
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Accused Products
Abstract
A nonhemolytic sensor for determination of analytes in erythrocyte-containing biological fluids is disclosed that comprises an optical material having a deposit on a surface thereof, where this deposit includes an analyte-reactive reagent and a particulate means for suppressing hemolysis of erythrocytes in an erythrocyte-containing biological fluid, such as whole blood. Thus, a porous array of negatively charged particles, e.g., polymeric beads having a plurality of carboxylate surface groups, are disposed on a sampling surface of an optical sensor, and suppress hemolysis of erythrocytes coming into contact with the sampling surface. In the case of an optical sensor, such particles can simultaneously enhance reflectance of a light beam transmitted through the optical material to the sampling surface site.
46 Citations
7 Claims
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1. A sensor for measuring concentration of an analyte in an erythrocyte-containing biological fluid comprising:
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an optical material having a surface site wherein the surface site of the optical material presents a textured field of projections and micro-wells, the projections being spaced apart for excluding the erythrocytes from entering into the micro-wells; and a deposit disposed on the surface site of the optical material for contacting the biological fluid, the deposit being disposed in each of the micro-wells and comprising an analyte-reactive reagent and an array of spherical styrene-acrylic beads penetrable to transport of the biological fluid exclusive of the erythrocytes contained therein. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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Specification