×

Method of measuring HbA1c

  • US 8,008,085 B2
  • Filed: 01/30/2008
  • Issued: 08/30/2011
  • Est. Priority Date: 01/30/2007
  • Status: Active Grant
First Claim
Patent Images

1. A method of measuring HbA1c, wherein the method comprises:

  • (A) collecting a hemoglobin-containing material from a subject and storing the collected hemoglobin-containing material for a period of at least one day;

    (B) reducing carbon dioxide bonded to hemoglobin in the hemoglobin-containing material stored in (A) so as to produce a hemoglobin-containing sample;

    (C) cleaving the hemoglobin in the hemoglobin-containing sample produced in (B) by applying a protease treatment to the hemoglobin-containing sample;

    (D) treating a glycated part of a hemoglobin fragment obtained from cleaving the hemoglobin in the hemoglobin-containing sample in (C) with fructosyl amine oxidase; and

    (E) determining an amount of HbA1c in the hemoglobin-containing sample by measuring a redox reaction between the glycated part and the fructosyl amine oxidase and calculating the amount of HbA1c based on a measurement of the redox reaction.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×