Factor VII: remodeling and glycoconjugation of Factor VII
First Claim
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1. A cell-free, in vitro method of forming a covalent conjugate of a Factor VIIa peptide, said peptide having the formula:
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Abstract
The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.
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Citations
87 Claims
- 1. A cell-free, in vitro method of forming a covalent conjugate of a Factor VIIa peptide, said peptide having the formula:
- 18. A cell-free, in vitro method of forming a covalent conjugate of a Factor VIIa peptide, said peptide having the formula:
- 24. A cell-free, in vitro method of forming a covalent conjugate of a Factor VIIa peptide, said peptide having the formula:
- 30. A cell-free, in vitro method of forming a covalent conjugate of a Factor VIIa peptide, said peptide having the formula:
- 36. A cell-free, in vitro method of forming a covalent conjugate of a Factor VIIa peptide, said peptide having the formula:
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46. A cell-free, in vitro method of forming a covalent conjugate between a poly(ethylene) glycol and a glycosylated or non-glycosylated Factor VIIa peptide, wherein said poly(ethylene) glycol is conjugated to said Factor VIIa peptide via a glycosyl linking group interposed between and covalently linked to both said Factor VIIa peptide and said poly(ethylene) glycol, said method comprising:
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(a) contacting said Factor VIIa peptide with a mixture comprising a nucleotide sugar having a urethane linker attached to said poly(ethylene) glycol, and a glycosyltransferase for which said nucleotide sugar is a substrate under conditions suitable for said at least one glycosyltransferase to transfer a modified sugar moiety of said nucleotide sugar onto said Factor VIIa peptide, wherein said modified sugar moiety comprises a urethane linker attached to at least one poly(ethylene) glycol, thereby forming said covalent conjugate of said Factor VIIa peptide. - View Dependent Claims (47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69)
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- 70. A cell-free, in vitro method of forming a covalent conjugate of a Factor VIIa peptide, said peptide having the formula:
- 76. A method of forming a covalent conjugate between a Factor VIIa peptide and a poly(ethylene) glycol, wherein said poly(ethylene) glycol is covalently attached to said Factor VIIa peptide through an intact glycosyl linking group, said Factor VIIa peptide comprising a glycosyl residue having a formula which is a member selected from:
Specification