Phosphonate fluorescent dyes and conjugates

• US 8,008,522 B2
• Filed: 01/06/2010
• Issued: 08/30/2011
• Est. Priority Date: 08/13/2004
• Status: Active Grant

• Current Assignee: Elitechgroup MDX LLC
• Original Assignee: Elitech Holding BV (Bruker Corporation)
• Inventors: Lukhtanov, Eugeny, Vorobiev, Alexei
• Primary Examiner(s): Katakam; Sudhakar
• Application Number:
  US12/683,305
• Publication Number:
  US 20100261903A1
• Time in Patent Office: 601 Days
• Field of Search: None
• US Class Current: 558/204
• CPC Class Codes: C07F 9/1411 with hydroxyalkyl compounds with further substituents on alkyl
• CPC Class Codes: C07F 9/5728 condensed with carbocyclic rings or carbocyclic ring systems
• CPC Class Codes: C07F 9/6533 Six-membered rings
• CPC Class Codes: C07F 9/6547 condensed with carbocyclic rings or carbocyclic ring systems
• CPC Class Codes: C07F 9/65522 condensed with carbocyclic rings or carbocyclic ring systems
• CPC Class Codes: C07F 9/65583 each of the hetero rings containing nitrogen as ring hetero atom
• CPC Class Codes: C07F 9/65586 at least one of the hetero rings does not contain nitrogen as ring hetero atom
• CPC Class Codes: C07F 9/6561 containing systems of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring or ring system, with or without other non-condensed hetero rings
• CPC Class Codes: C07F 9/65842 Cyclic amide derivatives of acids of phosphorus, in which one nitrogen atom belongs to the ring
• CPC Class Codes: C09B 11/08 Phthaleins; Phenolphthaleins; Fluorescein
• CPC Class Codes: C09B 11/24 Phthaleins containing amino groups ; Phthalanes; Fluoranes; Phthalides; Rhodamine dyes; Phthaleins having heterocyclic aryl rings; Lactone or lactame forms of triarylmethane dyes
• CPC Class Codes: C09B 23/083 five >CH- groups

Abstract

Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.

Claims

1. A compound having the formula:

2. A compound of claim 1, wherein X is —

NR^AR^B.

3. A compound of claim 1, wherein X is —

O-dimethyoxytrityl.

4. A compound of claim 1, selected from the group consisting of