Biosensor for disease diagnosis

• US 8,012,322 B2
• Filed: 03/18/2008
• Issued: 09/06/2011
• Est. Priority Date: 03/19/2007
• Status: Expired due to Fees

• Current Assignee: Yeongdong Dianostics Corporation, Yeongdong Electronic Co., Ltd.
• Original Assignee: Yeongdong Dianostics Corporation, Yeongdong Electronic Co., Ltd.
• Inventors: Kim, Myoung Kyu, Mok, Rak Sun, Park, Young Nam
• Primary Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US12/050,815
• Publication Number:
  US 20080277278A1
• Time in Patent Office: 1,267 Days
• Field of Search: 20440301-40315
• US Class Current: 204/403.04
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

The present invention relates to a biosensor including: a bottom plate having a base film having a lead mounted at one side of the top surface thereof, an electrode member formed on the base film, a spacer formed on the top surface of the electrode member so as to secure a recess having a predetermined width formed on the electrode member, and an enzyme reaction layer formed transversely on the top surface of the electrode member in such a fashion as to be positioned in the recess of the spacer; and a top plate formed integrally with the bottom plate and having the same configuration as the bottom plate.

Claims

1. A biosensor for disease diagnosis using an electrode, comprising:

a bottom plate including,

a first base film having a first lead mounted at one side of the top surface thereof,

a first electrode member formed on the first base film, one side of the first electrode member being the first lead,

a first spacer formed on the top surface of the first electrode member so as to secure a first recess having a predetermined width formed on the first electrode member, and

a first enzyme reaction layer formed transversely on the top surface of the first electrode member in such a fashion as to be positioned in the first recess of the first spacer; and

a top plate formed integrally with the bottom plate and including,

a second base film having a second lead mounted at one side of the top surface thereof,

a second electrode member formed on the second base film,

a second spacer formed on the top surface of the second electrode member so as to secure a second recess having a predetermined width formed on the second electrode member, and

a second enzyme reaction layer formed transversely on the top surface of the second electrode member in such a fashion as to be positioned in the second recess of the second spacer,

wherein the first and second base films are made of any one selected from heat-resistant polymers like polyethylene terephthalate (PET), polyethylene (PE) and polyvinyl chloride (PVC),

the first spacer and the second spacer contact each other, and

the first recess is formed transversely across the first base film from one lateral side of the first base film to an opposite lateral side of the first base film, and the second recess is formed transversely across the second base film from one lateral side of the second base film to an opposite lateral side of the second base film.

2. The biosensor according to claim 1, wherein the first and second electrode members are formed of a conductive material like carbon, copper, gold, silver, platinum or palladium.

3. The biosensor according to claim 1, wherein an enzyme reaction solution is pipetted on the recess formed on the spacer of each of the top plate and the bottom plate, thereby preventing the generation of variations between the top plate and the bottom plate.

4. The biosensor according to claim 1, wherein the first enzyme reaction layer and the second enzyme reaction layer are spaced apart from each other.

5. The biosensor according to claim 1, wherein the first lead and the second lead are spaced apart from each other.

6. The biosensor according to claim 1, wherein the first lead and the second lead are in different planes.

7. The biosensor according to claim 1, wherein the first lead includes two electrodes spaced apart from each other and the second lead includes two electrodes spaced apart from each other.

8. The biosensor according to claim 1, wherein the first lead is a portion of the first electrode protruding outwardly away from the first base film and the second lead is a portion of the second electrode protruding outwardly away from the second base film.

9. A biosensor for disease diagnosis using an electrode, comprising:

a bottom plate including,

a first base film having a first lead mounted at one side of the top surface thereof,

a first electrode member formed on the first base film, one side of the first electrode member being the first lead,

a first spacer formed on the top surface of the first electrode member so as to secure a first recess having a predetermined width formed on the first electrode member, and

a first enzyme reaction layer formed transversely on the top surface of the first electrode member in such a fashion as to be positioned in the first recess of the first spacer; and

a top plate formed integrally with the bottom plate and including,

a second base film having a second lead mounted at one side of the top surface thereof,

a second electrode member formed on the second base film,

a second spacer formed on the top surface of the second electrode member so as to secure a second recess having a predetermined width formed on the second electrode member, and

a second enzyme reaction layer formed transversely on the top surface of the second electrode member in such a fashion as to be positioned in the second recess of the second spacer,

wherein the first and second base films are made of any one selected from heat-resistant polymers like polyethylene terephthalate (PET), polyethylene (PE) and polyvinyl chloride (PVC),

the first spacer and the second spacer contact each other, and

the first enzyme reaction layer is formed transversely across the first base film from one lateral side of the first base film to an opposite lateral side of the first base film and is located entirely within the first recess, and the second enzyme reaction layer is formed transversely across the second base film from one lateral side of the second base film to an opposite lateral side of the second base film and is located entirely within the second recess.