Detection of target analytes using particles and electrodes

• US 8,012,743 B2
• Filed: 12/10/2001
• Issued: 09/06/2011
• Est. Priority Date: 10/27/1998
• Status: Expired due to Fees

• Current Assignee: Osmetech Technology Inc. (Roche Holding AG)
• Original Assignee: Osmetech Technology Inc. (Roche Holding AG)
• Inventors: Mucic, Robert C., Bamdad, Cynthia C.
• Primary Examiner(s): LU, FRANK WEI MIN
• Application Number:
  US10/016,416
• Publication Number:
  US 20110053788A1
• Time in Patent Office: 3,557 Days
• Field of Search: 435/6, 435/91.1, 435/183, 435/283.1, 435/287.1, 435/287.2, 436/94, 536/23.1, 536/24.3, 536/24.33, 536/25.3, 422/68.1
• US Class Current: 435/287.2
• CPC Class Codes: B01J 13/00 Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 2525/197 incorporating a spacer/coupling moiety
• CPC Class Codes: C12Q 2525/313 Branched oligonucleotides
• CPC Class Codes: C12Q 2563/113 the label being electroactive, e.g. redox labels
• CPC Class Codes: C12Q 2565/101 Interaction between at least two labels
• CPC Class Codes: C12Q 2565/1015 labels being on the same oligonucleotide
• CPC Class Codes: G01N 27/3275 Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
• CPC Class Codes: G01N 27/3277 being a redox reaction, e.g. detection by cyclic voltammetry voltammetry per se G01N27/42, G01N27/48
• CPC Class Codes: G01N 27/48 using polarography, i.e. measuring changes in current under a slowly-varying voltage
• CPC Class Codes: Y10S 977/773 Nanoparticle, i.e. structure having three dimensions of 100 nm or less
• CPC Class Codes: Y10S 977/92 Detection of biochemical
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

The invention relates to the use of particles comprising binding ligands and electron transfer moieties (ETMs). Upon binding of a target analyte, a particle and a reporter composition are associated and transported to an electrode surface. The ETMs are then detected, allowing the presence or absence of the target analyte to be determined.

Claims

1. A composition comprising:

a) a substrate comprising an array of working electrodes, wherein each electrode comprises a first binding ligand;

b) a plurality of colloids, each comprising;

i) a second binding ligand; and

ii) an electron transfer moiety; and

c) a detector capable of detecting a voltage associated with electron transfer from said electron transfer moiety.

2. The composition of claim 1 wherein said plurality of colloids further comprise a self-assembled monolayer.

3. The composition of claim 2 wherein said self-assembling monolayer comprises an alkyl chain.

4. The composition of claim 1 wherein said substrate is a printed circuit board.

5. The composition according to claim 1 wherein said electrodes are gold.

6. The composition according to claim 1 wherein said electron transfer moiety is a transition metal complex.

7. The composition according to claim 6 wherein said transition metal complex is ferrocene.

8. The composition according to claim 1 wherein said first binding ligand is a first nucleic acid and said second binding ligand is a second nucleic acid.