Thyroid analyte detection and measurement
First Claim
1. A non-radioisotopic method of detecting thyroid activity of a canine sample using a profile of thyroid analytes consisting of measuring T3, Free T3, T4, Free T4 and thyroglobulin autoantibody in a sample of a canine species and wherein each one of these analytes in an assay profile consists of a non-radio isotopic measurement of each of the analytes T3, Free T3, T4, Free T4 and thyroglobulin autoantibody in the sample from the canine species, and wherein the measurement of the T3, Free T3, T4, Free T4 and thyroglobulin autoantibody analytes in the profile collectively is indicative of thyroid activity.
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Abstract
A non-radioisotopic method of detecting thyroid analytes comprising detecting T3, Free T3, T4, Free T4 and thyroglobulin autoantibody in a sample of a non-human species. Each one of these analytes in an assay profile includes non-radio isotopic measurement of T3, Free T3, T4, Free T4 and thyroglobulin autoantibody in the sample from the non-human species. A non-radioisotopic method detects T3AA and T4AA thyroid autoantibodies in a sample from a non-human species such as the canine species. Antibodies and autoantibodies are bound, and a precipitated or bound antigen-antigen or antibody-autoantibody complex is formed. The supernatant or surrounding fluid of the bound or precipitated antigen-antigen or antibody-autoantibody complex is then removed. The thyroid activity of the bound complex, precipitate, supernatant or surrounding fluid is measured. The thyroid analyte is at least one of T3, Free T3, T4 or Free T4. A dialysis cell can be used to separate by dialysis the T3AA or T4AA from their respective T3AA or T4AA fractions without radioisotopes. A non-radioisotopic method detects Free T3 and/or Free T4 in a sample of a non-human species.
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Citations
14 Claims
- 1. A non-radioisotopic method of detecting thyroid activity of a canine sample using a profile of thyroid analytes consisting of measuring T3, Free T3, T4, Free T4 and thyroglobulin autoantibody in a sample of a canine species and wherein each one of these analytes in an assay profile consists of a non-radio isotopic measurement of each of the analytes T3, Free T3, T4, Free T4 and thyroglobulin autoantibody in the sample from the canine species, and wherein the measurement of the T3, Free T3, T4, Free T4 and thyroglobulin autoantibody analytes in the profile collectively is indicative of thyroid activity.
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14. A non-radioisotopic method of detecting thyroid activity of a canine sample using a profile of thyroid analytes consisting of measuring T3, Free T3, T4, Free T4 and thyroglobulin autoantibody in a sample of biological fluid from a canine species, and wherein each one of these analytes consists of measuring by a non-radioisotopic measurement for each of the analytes T3, Free T3, T4, and Free T4 and thyroglobulin autoantibody in the sample of biological fluid from the canine species, adding the sample to a non-radioisotopic luminescence reaction assay for measuring each of T3, Free T3, T4 and Free T4 and thyroglobulin antibody in the canine species, measuring the amount of non radioisotopic luminescence from a reaction of the sample with a reactant in the assay, and measuring thyroglobulin autoantibody in the sample by a non-radioisotopic immunoassay thereby determining the amount of each of T4, Free T4, T3 and Free T3 and thyroglobulin autoantibody in the canine species, and wherein the measurement of the T3, Free T3, T4, Free T4 and thyroglobulin autoantibody analytes in the profile collectively is indicative of thyroid activity.
Specification