Immersion sensor for measuring the concentration of an analyte with the help of an oxidase
First Claim
1. A method for measuring the concentration of at least one analyte with the aid of an analyte-specific oxidase, said method comprising:
- providing an immersion sensor, said immersion sensor comprising at least one enzyme region with the analyte-specific oxidase immobilized therein, two electrodes lying in parallel and terminating in the at least one enzyme region, at least one space for gaseous oxygen formed in between and bordering the electrodes and bordering the at least one enzyme region directly or via an oxygen-permeable material, and at least one channel leading from the at least one enzyme region to a surface of the immersion sensor which limits diffusion and contains water;
introducing said at least one analyte into said immersion sensor, wherein said at least one analyte diffuses into said at least one enzyme region via said at least one channel and wherein said at least one analyte reacts with said analyte-specific oxidase, consuming oxygen and producing volatile hydrogen peroxide;
measuring the concentration of the at least one analyte by measuring amperometrically the reaction of the at least one analyte with said analyte-specific oxidase with the immersion sensor; and
correlating the concentration of the at least one analyte with said hydrogen peroxide production.
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Abstract
The invention relates to measuring the concentration of analytes with the aid of an oxidase in an immersion sensor situated in a fluid or in a matrix containing fluid. Oxygen diffuses into the enzyme layer from within, from a gas-filled space connected to the atmosphere and/or to an oxygen reservoir. This enables oxygen saturation of the oxidase in a low-oxygen or oxygen-free medium and/or at high analyte concentrations. The analyte diffuses into the enzyme layer in a channel or a number of channels which contain(s) water and limit(s) diffusion, wherein the channel/channels on the surface of the sensor is/are filled with a protein-impermeable, hydrophilic matrix. By increasing the channel cross-section on the surface of the sensor and/or by connecting the channel/channels to a protein-impermeable, porous, hydrophilic layer on the surface of the sensor, the effect of outer deposits on the diffusion resistance of the analyte is reduced.
13 Citations
16 Claims
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1. A method for measuring the concentration of at least one analyte with the aid of an analyte-specific oxidase, said method comprising:
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providing an immersion sensor, said immersion sensor comprising at least one enzyme region with the analyte-specific oxidase immobilized therein, two electrodes lying in parallel and terminating in the at least one enzyme region, at least one space for gaseous oxygen formed in between and bordering the electrodes and bordering the at least one enzyme region directly or via an oxygen-permeable material, and at least one channel leading from the at least one enzyme region to a surface of the immersion sensor which limits diffusion and contains water; introducing said at least one analyte into said immersion sensor, wherein said at least one analyte diffuses into said at least one enzyme region via said at least one channel and wherein said at least one analyte reacts with said analyte-specific oxidase, consuming oxygen and producing volatile hydrogen peroxide; measuring the concentration of the at least one analyte by measuring amperometrically the reaction of the at least one analyte with said analyte-specific oxidase with the immersion sensor; and correlating the concentration of the at least one analyte with said hydrogen peroxide production. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A method for measuring the concentration of at least one analyte with the aid of an analyte-specific enzyme, said method comprising:
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providing an immersion sensor, said immersion sensor comprising at least one enzyme region with the analyte-specific enzyme therein, two electrodes lying in parallel and terminating in the at least one enzyme region, at least one space for gaseous oxygen formed in between and bordering the electrodes and bordering the at least one enzyme region directly or via an oxygen-permeable material, and at least one channel leading from the at least one enzyme region to a surface of the immersion sensor which limits diffusion and contains water; introducing said at least one analyte into said immersion sensor, wherein said at least one analyte diffuses into said at least one enzyme region via said at least one channel, and wherein said at least one analyte reacts with said analyte-specific enzyme, consuming oxygen; measuring the concentration of the at least one analyte by measuring amperometrically the reaction of the at least one analyte with said analyte-specific enzyme with the immersion sensor; and correlating the concentration of the at least one analyte with said oxygen consumption. - View Dependent Claims (15, 16)
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Specification