Seeding cells on porous supports
First Claim
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1. A method for seeding a suspension of a plurality of cells onto a porous hydrophobic support, wherein the pores are between about 50and about 1000 microns, comprising the steps of:
- a. Introducing a liquid media to the support,b. Partially removing the liquid media from the support by application of a centrifugal force, andc. After partially removing the liquid media, contacting the support with a suspension of a plurality of cells.
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Abstract
The present invention generally relates to a method for seeding cells on to a support. In particular, the method relates to a method for seeding cells onto a porous hydrophobic support. The method utilizes centrifugal forces to uniformly guide cell seeding into the support with no loss in viability.
7 Citations
1 Claim
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1. A method for seeding a suspension of a plurality of cells onto a porous hydrophobic support, wherein the pores are between about 50and about 1000 microns, comprising the steps of:
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a. Introducing a liquid media to the support, b. Partially removing the liquid media from the support by application of a centrifugal force, and c. After partially removing the liquid media, contacting the support with a suspension of a plurality of cells.
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Specification
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Current AssigneeLifescan Incorporated (Duv Holding Corporation)
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Original AssigneeLifescan Incorporated (Duv Holding Corporation)
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InventorsGhabrial, Ragae, Rezania, Alireza
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Primary Examiner(s)Ketter; James S
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Application NumberUS11/303,244Publication NumberTime in Patent Office2,097 DaysField of SearchNoneUS Class Current435/399CPC Class CodesC12N 2533/40 Polyhydroxyacids, e.g. poly...C12N 5/0068 General culture methods usi...
