Apparatus for microarray binding sensors having biological probe materials using carbon nanotube transistors

• US 8,017,938 B2
• Filed: 03/19/2007
• Issued: 09/13/2011
• Est. Priority Date: 03/17/2006
• Status: Expired due to Fees

• Current Assignee: Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services, University of Maryland, College Park
• Original Assignee: United States Department Of Health And Human Services (Government of the United States of America), University of Maryland, College Park
• Inventors: Aschenbach, Konrad, Wei, Jun Stephen, Pandana, Herman, Gomez, Romel Del Rosario, Khan, Javed, Fuhrer, Michael
• Primary Examiner(s): Gebremariam; Samuel
• Assistant Examiner(s): Arena; Andrew O
• Application Number:
  US11/723,369
• Publication Number:
  US 20080035494A1
• Time in Patent Office: 1,639 Days
• Field of Search: 257/9, 257/20, 257/40, 257/E51.001, 257/E51.002, 257/E51.003, 257/E51.004, 257/E51.005, 257/E51.006, 257/E51.023, 257/E51.024, 257/E51.025, 257/E51.026, 257/E51.038, 257/E51.039, 257/E51.04, 977/700, 977/701, 977/702, 977/703, 977/704, 977/705, 977/706, 977/785, 977/920, 977/921, 977/922, 977/936, 977/938
• US Class Current: 257/40
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: Y10S 977/702 having biological material component
• CPC Class Codes: Y10S 977/92 Detection of biochemical
• CPC Class Codes: Y10S 977/938 Field effect transistors, FETS, with nanowire- or nanotube-channel region

Abstract

A microarray apparatus is provided which contains at least one chip having source and drain electrodes positioned on an array of carbon nanotube transistors which allows for electronic detection of nucleic acid hybridizations, thereby affording both increased sensitivity and the capability of miniaturization.

Claims

1. An apparatus comprising:

one or more carbon nanotube transistors on a silicon substrate, the one or more carbon nanotube transistors each including a gate electrode, a source electrode, a drain electrode, and a carbon nanotube channel bridging the source electrode and the drain electrode, wherein the drain electrode and the carbon nanotube channel are covered by insulating layer, wherein the insulating layer insulates the drain electrode and the carbon nanotube channel from an electrolyte solution, wherein the gate electrode is configured to contact the electrolyte solution comprising one or more target biological molecules,

one or more specific probe materials immobilized on the insulating layer,

an electronic circuitry to detect a change in electrical charge by each of the one or more carbon nanotube transistors due to binding of the one or more specific probe materials with the one or more target biological molecules,

a detector to detect the change in electrical charge to directly quantify the amount of a bound target biological molecules; and

an automated sensing system for determining a relative abundance of the specific target biological molecule based on the change in electrical charge.

2. The apparatus of claim 1, wherein the one or more carbon nanotube transistors are made by electrically contacting a nanotube mat, and allowing an electrical current to flow through the nanotube mat in proximity to a third electrode separated by an insulating barrier where a voltage applied will cause an electric field to affect the conductance of the one or more carbon nanotube transistors.

3. The apparatus of claim 1, wherein the exposed metallic terminals form the electrical connection to the one or more carbon nanotube transistors.

4. The apparatus of claim 1, wherein the substrate is a glass or a non-conducting polymer.

5. The apparatus of claim 1, wherein the thin insulating layer is an oxide.

6. The apparatus of claim 1, wherein the one or more probe materials are chosen from DNA, RNA, PNA, antibodies, modified antibodies, proteins, peptides, aptamers, and peptide aptamers.

7. The apparatus of claim 1, wherein at least one of the exposed metallic terminals is located on the back side of the substrate.

8. The apparatus of claim 1, wherein the electronic circuitry is external to the at least one of the one or more carbon nanotube transistors.

9. The apparatus of claim 1, wherein the automated sensing system is external to the at least one of the one or more carbon nanotube transistors.

10. The apparatus of claim 1, wherein said at least one of the one or more carbon nanotube transistors is comprised on an array of about 20 to 50 carbon nanotube transistors.

11. The apparatus of claim 1, wherein the carbon nanotube channel bridges a gap between the source and the drain electrodes.

12. The apparatus of claim 1, wherein the multiplicity of specific probe materials comprise cell surface receptor sequences.

13. The apparatus of claim 1, wherein the drain electrode is gold.

14. The apparatus of claim 1, wherein a separation distance between the source and drain electrode is about 5 nm.

15. The apparatus of claim 1, further comprising:

a gate oxide on top of a drain source gap to avoid an electrolyte current leakage, wherein the gate oxide is not insulated by the thin insulating oxide or the nitride layer.

16. The apparatus of claim 15, wherein the gate oxide is an aluminum oxide.

17. The apparatus of claim 16, wherein the aluminum oxide is about 100 nm thick.

18. The apparatus of claim 1, wherein the specific probe materials are immobilized on the insulating oxide or the nitride layer by silane functionalization.

19. The apparatus of claim 18, wherein the silane functionalization comprises MPTMS coupled to a hydroxylated surface of the thin insulating oxide or the nitride layer.

20. A method of electronically detecting a target biological material in a mixture containing the target biological material and other biological materials, which comprises:

a) exposing the mixture containing the target biological material and other biological materials to the at least one chip of the apparatus of claim 1; and

b) determining an absolute amount of the target biological material in the mixture by a change in electrical charge due to binding of the target biological material to a probe material on said at least one chip.

21. The method of claim 20, which further comprises electronically determining a relative abundance of target biological materials in a mixture comprising more than one target biological material.

22. The method of claim 20, wherein the apparatus comprises one chip.

23. The method of claim 20, wherein the target biological material is DNA.

24. The method of claim 20, wherein the target biological material is RNA.

25. The method of claim 24, wherein the RNA is miRNA.

26. The method of claim 20, wherein the probe material is DNA, RNA or PNA.

27. The apparatus of claim 1, wherein the insulating layer comprises a thin insulating oxide or a nitride.

28. The apparatus of claim 27, wherein the thin insulating layer includes exposed metallic terminals for providing electrical conductivity.