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Integrated nucleic acid analysis

  • US 8,018,593 B2
  • Filed: 04/04/2008
  • Issued: 09/13/2011
  • Est. Priority Date: 04/04/2007
  • Status: Active Grant
First Claim
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1. An integrated biochip system comprising:

  • (a) a biochip comprising a plurality microfluidic systems, wherein each microfluidic system comprises a first reaction chamber in microfluidic communication with a separation chamber, whereinthe first reaction chamber is adapted for;

    (i) nucleic acid extraction;

    (ii) nucleic acid purification;

    (iii) pre-nucleic acid amplification cleanup;

    (iv) nucleic acid amplification;

    (v) post-nucleic acid amplification cleanup;

    (vi) pre-nucleic acid sequencing cleanup;

    (vii) nucleic acid sequencing;

    (viii) post-nucleic acid sequencing cleanup;

    (ix) reverse transcription;

    (x) pre-reverse transcription cleanup;

    (xi) post-reverse transcription cleanup;

    (xii) nucleic acid ligation;

    (xiii) nucleic acid hybridization;

    or(xiv) quantification; and

    the separation chamber comprises a detection position; and

    (b) a separation and detection system comprising,(i) a separation element for simultaneously separating a plurality of target analytes in the separation chamber;

    (ii) one or more light sources positioned to illuminate the detection positions on the biochip;

    (iii) a mirror to scan said one or more light sources sequentially between said detection positions;

    (iv) one or a plurality of first optical elements positioned for collecting and directing light emanating from the detection positions; and

    (v) a light detector positioned to accept light directed from the one or plurality of first optical elements, wherein the light detector comprises a wavelength dispersive element to disperse the light from the one or plurality of first optical elements according to light wavelength into at least 6 wavelength components and, the wavelength dispersive element is positioned to provide at least a portion of the dispersed at least 6 wavelength components to at least 6 detection elements, whereineach of the detection elements is in communication with a first control element for simultaneously collecting detection information from each of the detection elements; and

    wherein said light detector detects fluorescence from at least 6 dyes labeled to one or more biological molecules, each dye having a unique peak wavelength.

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