Biosensor

• US 8,021,528 B2
• Filed: 03/06/2008
• Issued: 09/20/2011
• Est. Priority Date: 03/07/2007
• Status: Active Grant

• Current Assignee: Yong-Sang Jang
• Original Assignee: Yong-Sang Jang
• Inventors: Jang, Yong-Sang
• Primary Examiner(s): Barton; Jeffrey T
• Assistant Examiner(s): DIETERLE, JENNIFER M
• Application Number:
  US12/043,375
• Publication Number:
  US 20080217171A1
• Time in Patent Office: 1,293 Days
• Field of Search: 20440301-40315, 205/775, 205/777.5, 205/778, 205/792, 600345-348, 422400-430, 422500-570
• US Class Current: 204/403.14
• CPC Class Codes: C12Q 1/004 mediator-assisted

Abstract

A biosensor that is capable of measuring a material contained in a sample is provided. The biosensor is configured to be inserted into a display device, and measures a material contained in the sample. The biosensor includes i) first and second substrates that are opposed to each other; ii) a sample guiding layer that has two sample injection openings and is located on the first substrate; iii) a first electrode that is located between the first substrate and the sample guiding layer; iv) a second electrode that is located between the second substrate and the sample guiding layer; v) a third electrode that is located between the sample guiding layer and the second substrate; and vi) a penetrated opening that penetrates the first substrate, the sample guiding layer, and the second substrate. The second electrode is spaced apart from the first electrode. The biosensor further includes i) a long edge, and ii) a short edge that shares a corner of the biosensor and neighbors the long edge. Each of the two sample injection openings is formed to correspond to the long edge and the short edge, respectively.

Claims

1. A biosensor that is configured to be inserted into a display device and measures a material contained in a sample, the biosensor comprising:

first and second substrates that are opposed to each other and comprise a long edge, and a short edge that shares a corner of the biosensor and neighbors the long edge;

a sample guiding layer;

a penetrated opening that penetrates the first substrate, the sample guiding layer, and the second substrate;

a first electrode that is located between the first substrate and the sample guiding layer;

a second electrode that is located between the first substrate and the sample guiding layer, and spaced apart from the first electrode;

a third electrode that is located between the sample guiding layer and the second substrate;

a fourth electrode that is located on the first substrate wherein the fourth electrode is located closer to the display device than the first electrode when the biosensor is inserted into the display device;

wherein the sample guiding layer has two sample inlets and is located on the first substrate, wherein each of the two sample inlets correspond to the long edge and short edge, respectively, and the sample inlet corresponding to the long edge is located closer to the fourth electrode than the penetrated opening;

wherein first and second sample guiding channels connect the two sample inlets, wherein the first and second guiding channels are connected to the penetrated opening at both sides of the penetrated opening opposite to each other; and

wherein the first guiding channel connects the sample inlet that corresponds to the short edge with the penetrated opening and the second guiding channel is bent and connects the sample inlet that corresponds to the long edge with the penetrated opening.

2. The biosensor of claim 1, wherein the display device comprises:

a first connector pin; and

a second connector pin that has a greater length than that of the first connector pin and is spaced apart from the first connector pin to be extended parallel to the first connector pin,

wherein the display device displays an error message when the first connector pin is connected to the fourth electrode and the second connector pin is connected to the first electrode.

3. The biosensor of claim 1, wherein the display device comprises:

a first connector pin; and

a second connector pin that has a greater length than that of the first connector pin and is spaced apart from the first connector pin to be extended parallel to the first connector pin,

wherein amount of the material is displayed when the first and second connector pins are electrically connected to the third electrode.

4. The biosensor of claim 1, wherein the at least one channel includes a plurality of channels and the channels are formed to extend parallel to each other,

wherein the first electrode comprises a first sample measuring unit that is formed between the channels to extend in a direction parallel to the channels, and

wherein the second electrode comprises a second sample measuring unit that is formed apart from the first sample measuring unit by a certain distance and is parallel thereto.

5. The biosensor of claim 1, wherein the first electrode comprises:

a first body portion;

a first connecting portion that is connected to the first body portion and neighbors the fourth electrode, the first connecting portion being configured to be electrically connected to the display device to be extended along a direction in which the biosensor is inserted into the display device; and

a sample contacting portion that is connected to the first connecting portion and is configured to contact the sample.

6. The biosensor of claim 5,

wherein the second electrode comprises two branched portions that meet with each of the two sample guiding channels,

wherein each of the two branched portions is spaced apart from the sample contacting portion, respectively, and

wherein each of the two branched portions is located to be closer to the hole than the sample contacting portion along the two sample guiding channels, respectively.

7. The biosensor of claim 5, wherein the third electrode comprises:

second body portion; and

second connecting portion that is connected to the second body portion, the second connecting portion being configured to be electrically connected to the display device and to be extended along a direction in which the biosensor is inserted into the display device,

wherein the first connecting portion and the second connecting portion are exposed to the outside in opposite directions to each other, and the second electrode is located between the first connecting portion and the second connecting portion.

8. The biosensor of claim 7, wherein the first connecting portion and the second connecting portion are located such that they are symmetrical to each other based on the second electrode along a direction that perpendicularly crosses the direction in which the biosensor is inserted into the display device.

9. The biosensor of claim 1, wherein the sample guiding layer further comprises a sample guiding channel that connects the sample inlet with the penetrated opening,

wherein a mediator is located in the sample guiding channel, the mediator comprising:

an enzyme that reacts with the material;

an electron transfer medium that transfers electrons generated from the enzyme; and

a dispersion stabilizer that disperses and stabilizes the enzyme and the electron transfer medium.

10. The biosensor of claim 9, wherein the enzyme is at least one selected from the group consisting of glucose oxidase, glucose dehydrogenase, alcohol oxidase, alcohol dehydrogenase, pyrroloquinone (PQQ), and nicotinamide adenine dinucleotide/hydrogen (NAD/NADH).

11. The biosensor of claim 9, wherein the electron transfer medium is at least one selected from the group consisting of ferrocene, quinone, cobalt, nickel, ruthenium, a ferricyan compound, rhodium, palladium, osmium, iridium, platinum, hexaamineruthenium(III) chloride, derivatives comprising these, and transition metals.

12. The biosensor of claim 9, wherein the dispersion stabilizer is at least one selected from the group consisting of polyvinyl alcohol, polyethylene oxide, polyethylene glycol, carboxymethyl cellulose, hydroxymethyl cellulose, 2-hydroxyethyl cellulose, hydroxypropyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, polyvinylidene fluoride, polymethyl methacrylate, and styrene butyl rubber.

13. The biosensor of claim 9, wherein the mediator further comprises a phase transfer catalyst, and

the phase transfer catalyst comprises at least one selected from the group consisting of phosphonium-based reagents, crown ether-based reagents, ammonium-based reagents, and polyethylene glycol (PEG)-based reagents.

14. The biosensor of claim 9, wherein the mediator further comprises glucose oxidase, hexaammineruthenium(III) chloride, carboxymethyl cellulose, microcrystalline cellulose, tricaprylmethyl ammonium chloride, t-octylphenoxypolyethoxyethanol, and a soap.

15. The biosensor of claim 9, wherein the mediator further comprises a surfactant,

wherein the surfactant comprises at least one selected from the group consisting of anionic surfactants, cationic surfactants, amphoteric surfactants, and nonionic surfactants.

16. The biosensor of claim 15, wherein the anionic surfactant comprises a soap or alkylbenzene sulfonate.