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Rapid and sensitive detection of cells and viruses

  • US 8,021,848 B2
  • Filed: 09/06/2002
  • Issued: 09/20/2011
  • Est. Priority Date: 09/06/2001
  • Status: Active Grant
First Claim
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1. A method for detecting individual target cells or viruses in a liquid sample, wherein said sample is added to a container comprising a detection surface and said targets measure less than 50 microns in at least two orthogonal dimensions,wherein said method comprises the steps of:

  • a. contacting said individual targets with one or more signaling moieties that specifically bind to the targets,b. depositing said targets on said detection surface so that they are randomly dispersed in a detection zone comprising a detection area at a density of less than 100 target cells per mm2,c. illuminating said individual targets to generate a detectable signal from said one or more signaling moieties, andd. simultaneously detecting said individual targets by detecting said signal in a section of said detection area using a photoelectric detector, wherein the longest linear dimension of said section is greater than 1 mm, using magnification of less than or equal to 5 times, wherein said one or more signaling moieties that do not bind to said targets are not removed from said container prior to said detecting.

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