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Methods of isolation, expansion and differentiation of fetal stem cells from chorionic villus, amniotic fluid, and placenta and therapeutic uses thereof

  • US 8,021,876 B2
  • Filed: 11/15/2002
  • Issued: 09/20/2011
  • Est. Priority Date: 11/15/2001
  • Status: Expired due to Fees
First Claim
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1. A method of proliferating a population of cells enriched for human pluripotent fetal stem cells comprising:

  • (a) selecting at least one c-kit positive cell from a human amniotic fluid sample, wherein said cell is c-kit, SSEA3 and SSEA4 positive, and immunonegative for SSEA1;

    (b) introducing said at least one selected cell to a culture medium; and

    (c) proliferating said at least one selected cell in the culture medium.

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