Method of measuring glycated hemoglobin concentration

• US 8,021,887 B2
• Filed: 03/23/2007
• Issued: 09/20/2011
• Est. Priority Date: 03/24/2006
• Status: Active Grant

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Sugiyama, Koji, Sakai, Toshikatsu
• Primary Examiner(s): Wallenhorst; Maureen M
• Application Number:
  US12/225,526
• Publication Number:
  US 20090317912A1
• Time in Patent Office: 1,642 Days
• Field of Search: 436/63, 436/66, 436/67, 436/161, 436/164, 436/174, 422/68.1, 422/70, 422/73, 422/82.05, 422/82.09
• US Class Current: 436/67
• CPC Class Codes: G01N 2021/3137 with selection of wavelengths after the sample
• CPC Class Codes: G01N 2021/3144 for oxymetry
• CPC Class Codes: G01N 2030/8822 involving blood
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/314 with comparison of measurements at specific and non-specific wavelengths dual wavelength spectrometry G01J3/427
• CPC Class Codes: G01N 30/74 Optical detectors measurement of intensity, velocity, spectral content, polarisation, or phase of infrared, visible or ultraviolet light G01J
• CPC Class Codes: G01N 30/88 Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
• CPC Class Codes: G01N 33/723 Glycosylated haemoglobin
• CPC Class Codes: Y10T 436/25 including sample preparation

Abstract

The present invention relates to a method and an apparatus that measure the concentration of glycated hemoglobin by an optical technique. A wavelength in which the molecular extinction coefficient of oxyhemoglobin agrees or substantially agrees with the molecular extinction coefficient of deoxyhemoglobin is adopted as a measurement wavelength. Preferably, the measurement wavelength is set at from 417 to 421 nm. In the present invention, the concentration of glycated hemoglobin is measured by making use of column chromatography and of using a sample prepared from red blood cells in blood.

Claims

1. A method of measuring the concentration of glycated hemoglobin in a sample, the method comprising the steps of:

irradiating the sample with light of a measurement wavelength, the measurement wavelength being set at about 423 nm,

irradiating the sample with light of a reference wavelength, and

calculating the concentration of glycated hemoglobin in the sample based on reception results of the measurement wavelength and correction data, the correction data being based on reception results of the reference wavelength.

2. The method of measuring the concentration of glycated hemoglobin according to claim 1, further comprising:

separating glycated hemoglobin by column chromatography so as to prepare the sample.

3. The method of measuring the concentration of glycated hemoglobin according to claim 2, further comprising:

preparing laked blood from red blood cells prior to the separating of the glycated hemoglobin.

4. The method of measuring the concentration of glycated hemoglobin according to claim 1, further comprising:

dividing light that travels through the sample into light of the measurement wavelength and light of the reference wavelength.

5. The method of measuring the concentration of glycated hemoglobin according to claim 4, further comprising:

receiving the light of the measurement wavelength that travels through the sample with a first light receiving section.

6. The method of measuring the concentration of glycated hemoglobin according to claim 5, further comprising:

receiving the light of the reference wavelength that travels through the sample with a second light receiving section.

7. An apparatus configured to measure the concentration of glycated hemoglobin, the apparatus comprising:

a photometry mechanism in which a sample is irradiated with light of a measurement wavelength, the measurement wavelength being set at about 423 nm, and light from a reference wavelength, wherein the photometry mechanism includes

a first light receiving section that receives light of the measurement wavelength that travels through the sample, and

a second light receiving section that receives light of the reference wavelength that travels through the sample; and

a calculating section that calculates the concentration of glycated hemoglobin in the sample based on reception results of the measurement wavelength and correction data, the correction data being based on reception results of the reference wavelength.

8. The apparatus according to claim 7, further comprising:

a separation unit configured to separate glycated hemoglobin by column chromatography so as to prepare the sample.

9. The apparatus according to claim 8, further comprising:

a sample preparation mechanism configured to prepare laked blood from red blood cells prior to separation of the glycated hemoglobin.

10. The apparatus according to claim 7, further comprising:

a beam splitter that divides the light of the measurement wavelength that travels through the sample from the light of the reference wavelength that travels through the sample.

11. The apparatus according to claim 7, wherein the second light receiving section is configured to receive light of the reference wavelength of about 500 nm.