Detection of nucleic acids from multiple types of human papillomaviruses
First Claim
1. A combination of oligonucleotides for detecting human papillomavirus (HPV) target sequences in multiple HPV types, the combination of oligonucleotides comprising amplification oligomers for amplifying a region of an E6/E7 gene from HPV, wherein the amplification oligomers are a first amplification oligonucleotide that is SEQ ID No. 38, its complement and/or RNA equivalent and one or more second amplification oligonucleotides comprising sequences selected from the group consisting of SEQ ID Nos. 42, 19, 21, 23, 25, 27, complements thereof and/or RNA equivalents thereof, wherein at least one of the amplification oligonucleotides may include a 5′
- promoter sequence that is non-complementary to an HPV target sequence.
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Abstract
Nucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (HPV) associated with cervical cancer. Methods for detecting multiple HPV types in biological specimens by amplifying HPV nucleic acid sequences in vitro and detecting the amplified products are disclosed.
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Citations
19 Claims
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1. A combination of oligonucleotides for detecting human papillomavirus (HPV) target sequences in multiple HPV types, the combination of oligonucleotides comprising amplification oligomers for amplifying a region of an E6/E7 gene from HPV, wherein the amplification oligomers are a first amplification oligonucleotide that is SEQ ID No. 38, its complement and/or RNA equivalent and one or more second amplification oligonucleotides comprising sequences selected from the group consisting of SEQ ID Nos. 42, 19, 21, 23, 25, 27, complements thereof and/or RNA equivalents thereof, wherein at least one of the amplification oligonucleotides may include a 5′
- promoter sequence that is non-complementary to an HPV target sequence.
- View Dependent Claims (2, 3, 4)
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5. A method of detecting the presence of human papillomavirus (HPV) nucleic acid present in a biological sample, comprising the steps of:
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contacting nucleic acid in a biological sample suspected of containing RNA of at least one of HPV types 33 and 58 with a combination of amplification oligonucleotides that specifically hybridize to and amplify a HPV target sequence in an E7 target region sequence, wherein said combination of amplification oligonucleotides includes as a first amplification oligonucleotide at least one of;
SEQ ID NO;
24, 26, 25 joined at its 5′
end to a promoter sequences that is non-complementary to an HPV target sequence, and 27 joined at its 5′
end to a promoter sequences that is non-complementary to an HPV target sequence combined with a second amplification oligonucleotide that is with an amplification oligomer having a nucleotide sequence that is SEQ ID NO;
38;amplifying a HPV target sequence from the target region sequence in at least one of HPV types 33 and 58 by using the amplification oligonucleotides and a nucleic acid polymerase in vitro to produce an HPV amplified product; and detecting the amplified product to indicate the presence in the sample of at least one of HPV types 33 and 58 present in the biological sample. - View Dependent Claims (6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification