Method for the extraction of aleurone from bran

• US 8,029,843 B2
• Filed: 08/20/2001
• Issued: 10/04/2011
• Est. Priority Date: 08/21/2000
• Status: Active Grant

• Current Assignee: Buhler AG (Buhler Holding AG)
• Original Assignee: Buhler AG (Buhler Holding AG)
• Inventors: Otto, Thomas, Behrens, Raimund, Bogoni, Carlo, Bohm, Arturo
• Primary Examiner(s): Chawla; Jyoti
• Application Number:
  US10/344,809
• Publication Number:
  US 20030175384A1
• Time in Patent Office: 3,697 Days
• Field of Search: 426/443, 426/455, 426/459, 426/460, 426/463, 426/464, 426/478, 426481-483, 426/518
• US Class Current: 426/459
• CPC Class Codes: A23L 33/105 Plant extracts, their artificial duplicates or their derivatives
• CPC Class Codes: A23L 7/115 Cereal fibre products, e.g. bran, husk
• CPC Class Codes: A23L 7/198 Dry unshaped finely divided cereal products, not provided for in groups A23L7/117 - A23L7/196 and A23L29/00, e.g. meal, flour, powder, dried cereal creams or extracts
• CPC Class Codes: C12Y 302/01004 Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
• CPC Class Codes: C12Y 302/01006 Endo-1,3(4)-beta-glucanase (3.2.1.6)
• CPC Class Codes: C12Y 302/01032 Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1-3-beta-xylanase
• CPC Class Codes: C12Y 302/01088 Beta-L-arabinosidase (3.2.1.88)

Abstract

The invention relates to a method for the preparation of aleurone from bran, in particular, wheat bran, for the extraction of aleuronic cells in particular from wheat grain, whereby the mainly aleurone-containing aleuronic components are separated from the mainly non-aleurone-containing non-aleuronic components in the bran and the aleurone-containing components are then isolated. The separation can be achieved by biochemical/enzymatic means and/or by mechanical-abrasive means. The subsequent isolation and extraction can be achieved by wet and/or dry separating methods.

Claims

1. Method for processing bran and extracting aleurone cells, wherein the aleurone-containing aleurone components are separated from the aleurone-free non-aleurone components in the bran, and the aleurone-containing components are then removed, and wherein the aleurone-containing aleurone components are separated from the aleurone-free non-aleurone components by a mechanical force selected from the group consisting of impact forces, collision forces and combinations thereof, and wherein a fraction comprising the aleurone components and non-aleurone components separated from one another and having particle sizes above 100 μ

m are graded and sorted into fractions by channel impeller air-classification.

2. Method according to claim 1, wherein the bran is first fragmented to a bran fragment size of about 400-800 μ

m.

3. Method according to claim 1, wherein the bran is first freed of endosperm.

4. Method according to claim 1, wherein grading or sorting is performed by a combination of channel impeller air-classification and at least one of zigzag air-classification and cross-flow air classification.

5. Method according to claim 1, wherein there is grading or sorting in an electrical field following grading or sorting by air-classification.

6. Method according to claim 1, wherein the bran is wheat bran and the aleurone cells are those of wheat kernels.

7. Method according to claim 1, wherein separation is carried out in an impact mill operating at a speed in the range of approximately 70 m/s to <

120 m/s.

8. Method according to claim 1, wherein separation is carried out such that no bending stress is applied to the bran while weight forces take effect on the bran.

9. The method according to claim 1, wherein the fraction comprising the aleurone components and non-aleurone components which is sorted into fractions by channel impeller air-classification has particle sizes above 100 μ

m and less than 300 μ

m.

10. Method according to claim 1, wherein the fine particles of a fine fraction extracted by air-classification are removed before they are subjected to additional grading by sieving.

11. Method according to claim 10, wherein grounded metallic sieves are used for sieving.

12. Method according to claim 1, wherein moisture and temperature during separation are controlled so as to allow optimal separation conditions.

13. Method according to claim 12, wherein the moisture is controlled in a range of between 8%-12% during separation, and wherein the temperature is controlled to not fall below 25°

C. during separation.

14. Application of the aleurone components separated or isolated by the method according to claim 1 as an additive in foodstuffs or feedstock, or as a food supplement or feed supplement.

15. Application according to claim 14, wherein the food supplement or the feed supplement is a pressed pellet of aleurone components and a nutritionally and physiologically harmless binding agent.

16. Application according to claim 14, wherein the additive or the food supplement or feed supplement is a drink.

17. Application according to claim 14, wherein the additive or the food supplement or feed supplement is a powder.

18. Application according to claim 14, wherein the food supplement is a starchy product.

19. Application according to claim 14, wherein the food supplement is a dairy product.

20. Aleurone-containing product containing the aleurone components separated or isolated according to claim 1.

21. Product according to claim 20, wherein the product is a powdered aleurone concentrate.

22. Product according to claim 20, wherein the product is an aleurone juice concentrate.

23. Method according to claim 1, wherein the aleurone tissues are separated from the seed coat undamaged by means of a mild mechanical action.

24. Method according to claim 23, wherein the mechanical action is applied to the bran in the form of active shearing forces.

25. Method according to claim 24, wherein the mechanical action is applied to the bran by means of an extruder.

26. Method according to claim 1, wherein separation is performed by mechanical and abrasive action.

27. Method according to claim 26, wherein separation of the aleurone components from the non-aleurone components is performed in a roller mill.

28. Method according to claim 26, wherein separation of the aleurone components from the non-aleurone components is performed in a centrifugal impact mill.

29. Method according to claim 26, wherein separation of the aleurone components from the non-aleurone components is performed in a jet mill.

30. Method according to claim 26, wherein separation of the aleurone components from the non-aleurone components is performed by a combination of the separation steps in claims 27 through 29.

31. Method according to claim 26, wherein the bran is moistened before it is subjected to the mechanical abrasive treatment.

32. Method according to claim 26, wherein the bran is added to water and the aleurone components are separated from the non-aleurone components in a colloid mill.

33. Method according to claim 26, wherein the bran is added to water and the aleurone components are separated from the non-aleurone components in a ball mill.

34. Method according to claim 33, wherein plastic balls are used in the ball mill.

35. Method according to claim 1, wherein the process is used to process wheat bran and whereby the different particles of the coarse fraction of the bran, which contains both non-aleurone hull particles originating in the furrow of the wheat kernel and aleurone particles, are electrically charged and/or polarized due to static electricity in various ways during their passage through the arched channel of the channel impeller air-classifier because of the particles rubbing against one another and/or the channel wall and they follow a path through an area subjected to an electrical field after leaving the channel impeller air-classifier, allowing the collection of the non-aleurone hull particles originating in the furrow at one spot and the collection of the aleurone particles at a second spot different from the first spot.

36. Method according to claim 35, wherein the arched channel is a circular channel.

37. Method according to claim 35, wherein the mill product consisting of ground bran is separated by simultaneously directing the mill product into an inclined, vibrating channel at one spot, whose inclination, surface roughness, and vibration are designed in such a way that the different mill product components move along the channel at different migration speeds, which allows different mill product fractions with more or fewer aleurone components to be extracted at different spots further along the channel at successive time intervals.

38. Method according to claim 37, wherein the separation of the mill product consisting of the ground bran by means of the channel is performed by simultaneously directing the mill product into an inclined, vibrating channel at one spot, whose inclination, surface roughness, and vibration are designed in such a way that the aleurone components migrate to the upper end of the channel while the non-aleurone components migrate to the lower.

39. Method according to claim 37, wherein the mill product is separated by sedimentation in a fluid-filled container, whereby different mill product fractions are extracted at the bottom of the container as sediment layers containing more or fewer aleurone components due to the differing sinking times of the various mill product components in the fluid-filled container.

40. Method according to claim 37, wherein the mill product is separated by moving the mill product particles along an equipotential surface of a heterogeneous electrical field, which causes the mill jet or the mill stream to be split into fractions containing more or fewer aleurone components due to the differing dielectric properties of the mill product particles.

41. Method according to claim 40, wherein the jet of mill product particles is moved by means of a laminar-flow carrier fluid flowing through an equipotential surface of the electrical field in a defined path and is thereby split by the heterogeneous electrical field.

42. Method according to claim 37, wherein the mill product particles become electrostatically charged by rubbing against one another and/or on part of the container and are then moved transversely to an electrical field, which causes the mill jet or the mill stream to be split into fractions containing more or fewer aleurone components due to the differing electrical properties of the mill product particles.

43. Method according to claim 42, wherein the jet of mill product particles is moved in a defined path by means of a laminar-flow carrier fluid flowing transversely to the electrical field and is then split by the electrical field.

44. Method according to claim 35, wherein separation is performed by a combination of the separation steps in claims 37 through 43.

45. Method according to claim 35, wherein the mill product fractions extracted are further treated with a biochemical substance specific to each fraction.

46. Method according to claim 45, wherein the biochemical substance contains at least one of the enzymes betaglucanase, cellulase, xylanase, and arabinase in a hydrated medium with which the mill product fractions are mixed into a hydrated mixture.

47. Method according to claim 45, wherein the biochemical substance contains at least one of the enzymes endoxylanase, beta-xylosidase, arabinofuranosidase, acetylesterase, xyloacetylesterase, and feruloyl esterase in a hydrated medium with which the mill product fractions are mixed into a hydrated mixture.

48. Method for processing bran and extracting aleurone cells, comprising the steps of:

separating aleurone-containing components from hull components in the bran by subjecting the bran to impact and/or collision forces in a mill;

sieving the bran including separate aleurone-containing components and hull components into fractions of different particle sizes including a fraction having a particle size of greater than 100 μ

m; and

air-classifying the fraction having particle sizes of greater than 100 μ

m by channel impeller air-classification to separate the aleurone-containing components from the hull components.

49. The method according to claim 48, wherein the air-classifying step is carried out on a fraction having particle sizes greater than 100 μ

m and less than 300 μ

m.