Method for preserving organs or limbs using a composition containing a siloxane and a semifluorinated alkane and/or hydrofluoroether

• US 8,029,977 B2
• Filed: 11/23/2006
• Issued: 10/04/2011
• Est. Priority Date: 11/23/2005
• Status: Expired due to Fees

• Current Assignee: Novaliq GmbH
• Original Assignee: Novaliq GmbH
• Inventors: Günther, Bernhard, Mühling, Bastian, Meinert, Hasso, Brandhorst, Daniel, Hiebl, Wilfried
• Primary Examiner(s): SAUCIER, SANDRA E
• Application Number:
  US12/094,951
• Publication Number:
  US 20090226875A1
• Time in Patent Office: 1,776 Days
• Field of Search: None
• US Class Current: 435/1.1
• CPC Class Codes: A01N 1/02 Preservation of living parts
• CPC Class Codes: A01N 1/0226 Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients

Abstract

Use of a composition which contains at least one semifluorinated alkane compound and at least one liquid siloxane, wherein the composition is of a density in a range of 0.8 to 1.5 g/cm³, for preserving organs or limbs.

Claims

1. A method of preserving an isolated organ or a limb comprising contacting the organ or limb with a water-free composition comprising at least one semifluorinated alkane or hydrofluoroether and at least one siloxane, wherein the composition has a density of 0.8 to 1.5 g/cm³, and wherein the semifluorinated alkane or hydrofluoroether and siloxane are liquid at ambient temperature, thereby preserving the organ or limb.

2. The method according to claim 1, wherein the semifluorinated alkane or hydrofluoroether has the formula R_FAR_H where R_F is a straight or branched perfluoroalkyl group with 1-20 C atoms, R_H is a straight or branched saturated alkyl group with 3-20 C atoms and A is a carbon to carbon bond or oxygen atom.

3. The method according to claim 2, wherein the at least one semifluorinated alkane or hydrofluoroether has branched units within the R_F group and/or within the R_H group.

4. The method according to claim 3, wherein the at least one semifluorinated alkane or hydrofluoroether can contain one or more branches designated X in the RF group, where X is CF₃, C₂F₅, C₃F₇ or C₄F₉ and can contain one or more side chains designated Y in the RH group, where Y is CH₃, C₂H₅, C₃H₇ or C₄H₉.

5. The method according to claim 2, wherein the semifluorinated alkane or hydrofluoroether RF is a straight or branched perfluoroalkyl group with 3-10 C atoms and RH is a straight or branched saturated alkyl group with 3-12 C atoms.

6. The method according to claim 1, wherein the composition contains a mixture of semifluorinated alkanes, a mixture of hydrofluoroethers or a combination of a least one semifluorinated alkane and at least one hydrofluoroether.

7. The method according to claim 1, wherein the siloxane is a dialkysiloxane and/or a diarylsiloxane, wherein the alkyl residues in the dialkylsiloxane can be straight-chain or branched and have 1-6 carbon atoms.

8. The method according to claim 1, wherein the siloxane is of a viscosity of 0.5 to 1000 mPas.

9. The method according to claim 8 wherein the siloxane is of a viscosity in the range of 0.5 to 500 mPas.

10. The method according to claim 1, wherein the siloxane is a polydimethylsiloxane or a mixture of polydimethylsiloxanes.

11. The method according to claim 1, wherein the composition has an oxygen content which corresponds to the oxygen demand of the organ or limbs to be preserved.

12. The method according to claim 1, wherein the composition further contains 10 to 40% by volume of oxygen, measured at ambient temperature.

13. The method according to claim 1, wherein the composition is saturated with oxygen at ambient temperature.

14. The method according to claim 1, wherein the composition additionally includes one or more active substances and/or nutrients.

15. The method according to claim 14, wherein the active substance or nutrient is an antibiotic, steroid, anti-inflammatory, cytostatic, virustatic and/or stabilizing agent.

16. The method according to claim 14, wherein the active substance or nutrient is 5-fluorouracil, daunomycin, aciclovir, ganciclovir, ibuprofen, N-acetylcysteine, carotenoids, retinol palmitate and/or α

-tocopherol.

17. The method according to claim 14, wherein the active substance or nutrient is a vitamin, lipid-bearing substance and/or easily decomposable fat and/or oil.

18. The method according to claim 14, wherein the composition further comprises a co-solvent for the active substance and/or nutrient.

19. The method according to claim 1, wherein the composition additionally contains at least one anti-oxidant.

20. The method according to claim 19 wherein the anti-oxidant is retinol palmitate and or α

-tocopherol.

21. The method according to claim 1, wherein the density of the composition is 0.9 to 1.3 g/cm³.

22. The method according to claim 1, wherein the density of the composition is 1.01 to 1.25 g/cm³.

23. A method of preserving an isolated organ or a limb comprising contacting the organ or limb with a water-free composition consisting essentially of at least one semifluorinated alkane or hydrofluoroether and at least one siloxane, wherein the composition has a density of 0.8 to 1.5 g/cm³, and wherein the semifluorinated alkane or hydrofluoroether and siloxane are liquid at ambient temperature, thereby preserving the organ or limb.