Antibodies to troponin I and methods of use thereof

• US 8,030,026 B2
• Filed: 02/24/2009
• Issued: 10/04/2011
• Est. Priority Date: 02/24/2009
• Status: Expired

• Current Assignee: Abbott Laboratories
• Original Assignee: Abbott Laboratories
• Inventors: Tu, Bailin, Huang, Dagang, Ziemann, Robert N., Brophy, Susan E., Tyner, Joan D.
• Primary Examiner(s): Shafer; Shulamith H
• Application Number:
  US12/391,937
• Publication Number:
  US 20100216720A1
• Time in Patent Office: 952 Days
• Field of Search: None
• US Class Current: 435/69.1
• CPC Class Codes: A61P 9/10 for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
• CPC Class Codes: C07K 14/4716 Muscle proteins, e.g. myosin, actin
• CPC Class Codes: C07K 16/18 against material from animals or humans
• CPC Class Codes: C07K 2317/24 containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
• CPC Class Codes: C07K 2317/565 Complementarity determining region [CDR]
• CPC Class Codes: C07K 2317/622 Single chain antibody (scFv)
• CPC Class Codes: C07K 2317/92 Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value

Abstract

The subject invention relates to antibodies to troponin I as well as methods of use thereof. In particular, such antibodies may be used to detect Troponin I in a patient and may also be used in the diagnosis of, for example, a myocardial infarction or acute coronary syndrome.

Claims

1. A Chinese Hamster Ovary (CHO) cell line, referred to as TnI 19C7 AM1 hG1 CHO 204 , designated by American Type Culture Collection (ATCC) deposit Number PTA-9816.

2. An isolated nucleic acid molecule encoding a binding protein, wherein the binding protein comprises a variable heavy chain, and wherein the amino acid sequence of the variable heavy chain of said binding protein has at least 90% identity to SEQ ID NO:

25 and wherein said binding protein maintains the binding activity of SEQ ID NO;

25.

3. The isolated nucleic acid molecule of claim 2, wherein the encoded binding protein further comprises a variable light chain, and wherein the amino acid sequence of the variable light chain of said binding protein has at least 90% identity to SEQ ID NO:

28 and wherein said binding protein maintains the binding activity of SEQ ID NO;

28.

4. An isolated nucleic acid molecule encoding a binding protein, wherein the binding protein comprises a variable heavy chain, and wherein the amino acid sequence of the variable heavy chain of said binding protein is SEQ ID NO:

25.

7. A vector comprising said isolated nucleic acid molecule of claim 4 claim 5.

8. An isolated host cell comprising said vector of claim 7.

9. A method of producing a binding protein capable of binding to Troponin I, comprising culturing said host cell of claim 8 for a time and under conditions sufficient to produce said binding protein.

5. An isolated nucleic acid molecule encoding a binding protein, wherein the binding protein comprises a variable light chain, and wherein the amino acid sequence of the variable light chain of said binding protein is SEQ ID NO:

28.

6. The isolated nucleic acid molecule of claim 5, wherein said encoded binding protein further comprises a variable heavy chain, and wherein the amino acid sequence of the variable heavy chain of said binding protein is SEQ ID NO:

25.