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Controlled electrophoresis method

  • US 8,030,092 B2
  • Filed: 11/17/2006
  • Issued: 10/04/2011
  • Est. Priority Date: 11/07/2003
  • Status: Active Grant
First Claim
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1. A controlled electrophoresis method, comprising:

  • introducing a sample into a transport passage of a multi-dimensional electrophoresis apparatus;

    the multi-dimensional electrophoresis apparatus including a first separation passage intersecting the transport passage at a first overlapping portion, a first analyte concentrator at the first overlapping portion to concentrate a first analyte of interest, a second separation passage intersecting the transport passage at a second overlapping portion, and a second analyte concentrator at the second overlapping portion to concentrate a second analyte of interest;

    passing the introduced sample in the transport passage into the first overlapping portion so that the first analyte of interest in the sample is concentrated in the first analyte concentrator;

    passing the introduced sample in the transport passage into the second overlapping portion so that the second analyte of interest in the sample is concentrated in the second analyte concentrator;

    localizing the first analyte concentrator and thereby increasing the concentration of the first analyte of interest by the first analyte concentrator;

    localizing the second analyte concentrator and thereby increasing the concentration of the second analyte of interest by the second analyte concentrator;

    releasing the first analyte of interest concentrated in the first analyte concentrator into the first separation passage;

    releasing the second analyte of interest concentrated in the second analyte concentrator into the second separation passage;

    delivering the released first analyte of interest in the first separation passage to an analyte detection zone which identifies and characterizes the first analyte of interest; and

    delivering the released second analyte of interest in the second separation passage to the analyte detection zone which also identifies and characterizes the second analyte of interest wherein;

    an intersection of the transport passage and the first separation passage has a staggered configuration such that the first overlapping portion is an elongated passage portion of the first separation passage and contains the first analyte concentrator; and

    /oran intersection of the transport passage and the second separation passage has a staggered configuration such that the second overlapping portion is an elongated passage portion of the second separation passage and contains the second analyte concentrator.

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