Anti-hedgehog antibodies

• US 8,030,454 B2
• Filed: 12/22/2008
• Issued: 10/04/2011
• Est. Priority Date: 12/28/2007
• Status: Active Grant

• Current Assignee: Genentech, Inc. (Roche Holding AG)
• Original Assignee: Genentech, Inc. (Roche Holding AG)
• Inventors: Scales, Suzanna J.
• Primary Examiner(s): Rawlings; Stephen
• Application Number:
  US12/341,910
• Publication Number:
  US 20100003239A1
• Time in Patent Office: 1,016 Days
• Field of Search: 530/350, 530/387.1, 530/387.3, 530/387.7, 530/388.1, 530/389.1
• US Class Current: 530/387.3
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 37/04 Immunostimulants
• CPC Class Codes: C07K 16/18 against material from animals or humans
• CPC Class Codes: G01N 33/57419 of colon
• CPC Class Codes: G01N 33/57449 of ovaries
• CPC Class Codes: G01N 33/57496 involving intracellular compounds

Abstract

The invention relates to anti-hedgehog antibodies, their use in the detection of hedgehog expression in tissue, and to the use of such detection in the treatment of cancer.

Claims

1. An anti-human sonic hedgehog antibody comprising a heavy chain and a light chain, wherein the heavy chain comprises the antigen binding regions HCHVR1 comprising the amino acid sequence of SEQ ID NO:

2, HCHVR2 comprising the amino acid sequence of SEQ ID NO;

4 and HCHVR3 comprising the amino acid sequence of SEQ ID NO;

6 and the light chain comprises the antigen binding regions LCHVR1 comprising the amino acid sequence of SEQ ID NO;

10, LCHVR2 comprising the amino acid sequence of SEQ ID NO;

12 and LCHVR3 comprising the amino acid sequence of SEQ ID NO;

14.

2. A monoclonal anti-human sonic hedgehog antibody that binds human sonic hedgehog at an epitope contained within the region of the protein having the amino acid sequence of amino acids 75-96 of SEQ ID NO:

16.

3. An article of manufacture for measuring human sonic hedgehog expression in a tissue sample of a patient, comprising the anti-human sonic hedgehog antibody of claim 2, and instructions for determining if human sonic hedgehog is overexpressed in a tissue, which instructions comprise the steps of contacting said tissue with said anti-human sonic hedgehog antibody and measuring the extent of binding.

4. The article of manufacture of claim 3, wherein the tissue is a tumor or cancer.

5. The article of manufacture of claim 3, wherein the tissue sample is removed from the patient prior to the determination.

6. The article of manufacture of claim 5, wherein the tissue sample is formalin fixed paraffin-embedded prior to contacting it with the anti-human sonic hedgehog antibody.

7. The article of manufacture of claim 3, wherein said instructions for determining if human sonic hedgehog is overexpressed in a tissue indicate that the determination is performed using a method selected from the group consisting of:

immunohistochemistry and Western blot.

8. The article of manufacture of claim 3, wherein the anti-human sonic hedgehog antibody does not compete with human ptch for binding to human sonic hedgehog.