Feline probiotic bifidobacteria

• US 8,034,601 B2
• Filed: 05/31/2006
• Issued: 10/11/2011
• Est. Priority Date: 05/31/2005
• Status: Active Grant

• Current Assignee: Mars Incorporated
• Original Assignee: The Procter & Gamble Company
• Inventors: Sunvold, Greg Dean, Boileau, Thomas William-Mawell, MacSharry, John, Kiely, Barry Pius, O'Mahony, Liam Diarmuid
• Primary Examiner(s): WARE, DEBORAH K
• Application Number:
  US11/443,765
• Publication Number:
  US 20060269534A1
• Time in Patent Office: 1,959 Days
• Field of Search: None
• US Class Current: 435/252.1
• CPC Class Codes: A23C 19/0323 using only lactic acid bacteria, e.g. Pediococcus and Leuconostoc species; Bifidobacteria; Microbial starters in general using moulds A23C19/0682
• CPC Class Codes: A23C 9/1234 characterised by using a Lactobacillus sp. other than Lactobacillus Bulgaricus, including Bificlobacterium sp.
• CPC Class Codes: A23K 10/18 of live microorganisms
• CPC Class Codes: A23K 50/40 for carnivorous animals, e.g. cats or dogs
• CPC Class Codes: A23K 50/42 Dry feed
• CPC Class Codes: A23K 50/48 Moist feed
• CPC Class Codes: A61K 35/745 Bifidobacteria
• CPC Class Codes: A61P 1/12 Antidiarrhoeals
• CPC Class Codes: A61P 1/14 Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61P 31/00 Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12R 2001/01 Bacteria or Actinomycetales ; using bacteria or Actinomycetales
• CPC Class Codes: Y10S 435/822 using bacteria or actinomycetales

Abstract

According to the invention there is provided a strain of lactic acid bacteria of the genus Bifidobacteria obtainable by isolation from resected and washed feline gastrointestinal tract having a probiotic activity in animals. Methods of use and compositions comprising the Bifidobacteria of the present invention are also provided.

Claims

1. A biologically pure strain isolated from resected and washed feline gastrointestinal tract having probiotic activity selected from the group consisting of Bifidobacterium lonqum, Bifidobacterium animalis, Bifidobacterium adolescentis, Bifidobacterium bifidum, Bifidobacterium infantis and Bifidobacterium thermophilum, said strain consisting of the 16s-23s intergenic polynucleotide sequence that has at least 95% homology to the DNA sequence of SEQ ID NO. 1 or at least 94% homology to the DNA sequence of SEQ. ID NO. 2.

2. The strain according to claim 1 having the ability to survive and colonise the gastrointestinal tract of a companion animal.

3. The strain according to claim 2 having at least 66% growth when cultured in the presence of 0.5% bile salts.

4. The strain according to claim 2 able to maintain viability following 1 hour at pH 2.5.

5. The strain according to claim 1 wherein the strain is of the species Bifidobacterium longum.

6. The strain according to claim 1, wherein said strain is selected from the group consisting of Bifidobacterium longum NCIMB 41290 (AHF5340) and Bifidobacterium longum NCIMB 41291 (AHF1231).

7. The strain according to claim 2 wherein the companion animal is a cat.