Methods of donor specific crossmatching
First Claim
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1. A method of cross-matching a donor and a recipient comprising:
- (a) providing a donor sample containing, or suspected of containing, a precursor endothelial cell;
(b) contacting the donor sample with a detection reagent to isolate the endothelial cell under conditions where the precursor endothelial cell and the detection reagent are capable of forming a complex;
(c) separating said complex from the sample, thereby isolating said precursor endothelial cell from said biological sample;
wherein the isolated precursor endothelial cell is CD34 negative and CD14 positive; and
(d) screening a recipient sample for a reactivity with the isolated precursor endothelial cell wherein no reactivity of the recipient sample with the isolated precursor endothelial cell indicates compatibility between the donor and recipient.
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Abstract
The detection of endothelial cell antibodies has been proven clinically important for successful organ transplantation. Disclosed are methods of isolating endothelial cell antibodies and methods for donor-specific crossmatching.
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Citations
10 Claims
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1. A method of cross-matching a donor and a recipient comprising:
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(a) providing a donor sample containing, or suspected of containing, a precursor endothelial cell; (b) contacting the donor sample with a detection reagent to isolate the endothelial cell under conditions where the precursor endothelial cell and the detection reagent are capable of forming a complex; (c) separating said complex from the sample, thereby isolating said precursor endothelial cell from said biological sample; wherein the isolated precursor endothelial cell is CD34 negative and CD14 positive; and (d) screening a recipient sample for a reactivity with the isolated precursor endothelial cell wherein no reactivity of the recipient sample with the isolated precursor endothelial cell indicates compatibility between the donor and recipient. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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Specification