Parallel preparation of high fidelity probes in an array format
First Claim
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1. A method for massively parallel oligonucleotide probe synthesis and release of the synthesized oligonucleotides from an array of probes on a solid substrate, the method comprising:
- providing a solid substrate;
attaching a plurality of linkers to the substrate, each linker comprising a cleavable moiety, wherein the cleavable moiety is activatable only at a distinct set of conditions and wherein activation of the cleavable moiety disrupts the linker to allow release of the synthesized oligonucleotides and to provide a substrate with a plurality of attached linkers, wherein the linker has the structure shown below
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Abstract
The present invention provides massively parallel oligonucleotide synthesis and purification for applications that utilize large collections of defined high-fidelity oligonucleotides (e.g., from about 101 to about 105 different sequences, generally between 25-160 bases in length).
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18 Claims
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1. A method for massively parallel oligonucleotide probe synthesis and release of the synthesized oligonucleotides from an array of probes on a solid substrate, the method comprising:
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providing a solid substrate; attaching a plurality of linkers to the substrate, each linker comprising a cleavable moiety, wherein the cleavable moiety is activatable only at a distinct set of conditions and wherein activation of the cleavable moiety disrupts the linker to allow release of the synthesized oligonucleotides and to provide a substrate with a plurality of attached linkers, wherein the linker has the structure shown below - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification