Methods for forming dyed microspheres and populations of dyed microspheres
First Claim
1. A method, comprising activating a chemical structure coupled to a dye using heat or light to form a reaction intermediate in the presence of a microsphere, wherein the reaction intermediate covalently attaches to a polymer of the microsphere thereby coupling the dye to the polymer and forming a dyed microsphere, and wherein the chemical structure comprises 1,5-ethyl-di-amino-naphthalene-sulfonic azide.
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Abstract
Various methods for forming dyed microspheres are provided. One method includes activating a chemical structure coupled to a dye using heat or light to form a reaction intermediate in the presence of a microsphere. The reaction intermediate covalently attaches to a polymer of the microsphere thereby coupling the dye to the polymer and forming the dyed microsphere. Additional methods are provided for forming a dyed microsphere coupled to a molecule. These methods include dyeing the microspheres as described above in addition to synthesizing the molecule on an outer surface of the dyed microspheres. A population of dyed microspheres is also provided. Each of the dyed microspheres of the population includes a dye attached to a polymer of each of the dyed microspheres by a chemical structure. A coefficient of variation in dye characteristics of the population of dyed microspheres attributable to the dye is less than about 10%.
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41 Claims
- 1. A method, comprising activating a chemical structure coupled to a dye using heat or light to form a reaction intermediate in the presence of a microsphere, wherein the reaction intermediate covalently attaches to a polymer of the microsphere thereby coupling the dye to the polymer and forming a dyed microsphere, and wherein the chemical structure comprises 1,5-ethyl-di-amino-naphthalene-sulfonic azide.
- 24. A method, comprising heating a chemical structure coupled to a dye in the presence of a microsphere to a temperature below a glass transition temperature of a polymer of the microsphere, wherein the step of heating the chemical structure forms a reaction intermediate which covalently attaches to the polymer thereby coupling the dye to the polymer and forming a dyed microsphere.
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