Assays employing randomly distributed microbeads with attached biomolecules
First Claim
1. A method of identifying targets capable of binding with bioactive agents, where the targets are contained in fluid samples, and wherein different types of bioactive agent are attached to differently-encoded microspheres, comprising:
- a) providing a substrate having said microspheres randomly distributed thereon, wherein the substrate surface comprises a plurality of assay locations, each assay location comprising an array location, said array locations comprising a plurality of discrete sites such that the microspheres form planar crystalline arrays;
b) contacting the microspheres with the fluid samples; and
c) contacting said microspheres with a plurality of different decoder binding ligands, whereby the location of said bioactive agents is identified and the targets in the fluid samples which bound to said bioactive agents are identified.
3 Assignments
0 Petitions
Accused Products
Abstract
A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relics on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations. In addition, the present invention provides a procedure for the creation of material surfaces with desired properties and for the fabrication of surface-mounted optical components.
163 Citations
18 Claims
-
1. A method of identifying targets capable of binding with bioactive agents, where the targets are contained in fluid samples, and wherein different types of bioactive agent are attached to differently-encoded microspheres, comprising:
-
a) providing a substrate having said microspheres randomly distributed thereon, wherein the substrate surface comprises a plurality of assay locations, each assay location comprising an array location, said array locations comprising a plurality of discrete sites such that the microspheres form planar crystalline arrays; b) contacting the microspheres with the fluid samples; and c) contacting said microspheres with a plurality of different decoder binding ligands, whereby the location of said bioactive agents is identified and the targets in the fluid samples which bound to said bioactive agents are identified. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
-
Specification