B7-DC variants
First Claim
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1. An isolated variant B7-DC polypeptide comprising an IgV domain, wherein the variant polypeptide is a variant of wild-type B7-DC polypeptide, has altered binding affinity for PD-1 compared to the wild-type B7-DC polypeptide, and comprises a substitution of an amino acid in the A′
- , B, C, C′
, C″
, D, E, F, or G β
-strand of wild-type B7-DC polypeptide according to SEQ ID NO;
5 or 6.
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Abstract
Variant costimulatory polypeptides, nucleic acids encoding such polypeptides, and methods for using the polypeptides and nucleic acids to enhance a T cell response are provided herein.
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Citations
57 Claims
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1. An isolated variant B7-DC polypeptide comprising an IgV domain, wherein the variant polypeptide is a variant of wild-type B7-DC polypeptide, has altered binding affinity for PD-1 compared to the wild-type B7-DC polypeptide, and comprises a substitution of an amino acid in the A′
- , B, C, C′
, C″
, D, E, F, or G β
-strand of wild-type B7-DC polypeptide according to SEQ ID NO;
5 or 6. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41)
- , B, C, C′
-
22. An isolated polypeptide fragment of murine B7-DC (SEQ ID NO:
- 6) or human B7-DC (SEQ ID NO;
5), the polypeptide fragment comprising amino acids 67-71, amino acids 101-105, or amino acids 111-113 of SEQ ID NO;
6, wherein the polypeptide inhibits binding of endogenous B7-DC to PD-1. - View Dependent Claims (23, 24, 25, 26, 27, 28)
- 6) or human B7-DC (SEQ ID NO;
-
42. A fusion polypeptide comprising the IgV domain of B7-DC fused to a second polypeptide, wherein the IgV domain of B7-DC is from human (SEQ ID NO:
- 5) or murine B7-DC (SEQ ID NO;
6) and comprises an amino acid substitution relative to wildtype human (SEQ ID NO;
5) or murine (SEQ ID NO;
6) B7-DC IgV domain, wherein the substitution alters binding affinity for PD-1 compared to the wild-type B7-DC polypeptide. - View Dependent Claims (43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57)
- 5) or murine B7-DC (SEQ ID NO;
Specification