Compensator for multiple surface imaging
First Claim
1. A method for imaging a biological sample, comprising:
- (a) detecting radiation emitted from a first emissive component of a biological sample disposed on a first surface of a flow cell using a detector, wherein the flow cell is mounted on an imaging station;
(b) inserting corrective optics between the detector and the flow cell;
(c) detecting radiation emitted from a second emissive component of a biological sample disposed on a second surface of the flow cell using the detector and the corrective optics, wherein the first and second surfaces are in an arrangement whereby one of the surfaces is disposed between the detector and the other surface, wherein the corrective optics reduce aberration of detection at one of the surfaces due to the arrangement; and
(d) repeating steps (a)-(c) while maintaining the flow cell on the imaging station.
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Accused Products
Abstract
A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.
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Citations
27 Claims
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1. A method for imaging a biological sample, comprising:
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(a) detecting radiation emitted from a first emissive component of a biological sample disposed on a first surface of a flow cell using a detector, wherein the flow cell is mounted on an imaging station; (b) inserting corrective optics between the detector and the flow cell; (c) detecting radiation emitted from a second emissive component of a biological sample disposed on a second surface of the flow cell using the detector and the corrective optics, wherein the first and second surfaces are in an arrangement whereby one of the surfaces is disposed between the detector and the other surface, wherein the corrective optics reduce aberration of detection at one of the surfaces due to the arrangement; and (d) repeating steps (a)-(c) while maintaining the flow cell on the imaging station. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. An imaging system for detecting radiation on a multi-surface flow cell, comprising:
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a multi-surface flow cell having first and second emissive components of a biological sample disposed on respective first and second surfaces of the flow cell; an optical train comprising an objective, imaging optics configured to focus the optical train on the first emissive component via the objective, and corrective optics configured to focus the optical train on the second emissive component and configured to reduce aberration of detection at the first or second emissive component; a radiation source configured to direct excitation radiation towards the first and second emissive components; detection optics configured to capture emitted radiation returned from the first and second emissive components via the optical train; and a detector for detecting the captured radiation. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27)
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Specification