Compensator for multiple surface imaging

• US 8,039,817 B2
• Filed: 05/01/2009
• Issued: 10/18/2011
• Est. Priority Date: 05/05/2008
• Status: Active Grant

• Current Assignee: Illumina Incorporated
• Original Assignee: Illumina Incorporated
• Inventors: Bryant, Jason, Buermann, Dale, Feng, Wenyi
• Primary Examiner(s): Kim; Kiho
• Application Number:
  US12/434,495
• Publication Number:
  US 20090272914A1
• Time in Patent Office: 900 Days
• Field of Search: 250/259.1, 250/458.1, 250/576
• US Class Current: 250/459.1
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 2021/6419 Excitation at two or more wavelengths
• CPC Class Codes: G01N 2021/6421 Measuring at two or more wavelengths
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/6458 Fluorescence microscopy fluorescence microscopes per se G02B21/0076 and G02B21/16
• CPC Class Codes: G02B 21/0024 Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
• CPC Class Codes: G02B 2207/113 Fluorescence

Abstract

A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

Claims

1. A method for imaging a biological sample, comprising:

(a) detecting radiation emitted from a first emissive component of a biological sample disposed on a first surface of a flow cell using a detector, wherein the flow cell is mounted on an imaging station;

(b) inserting corrective optics between the detector and the flow cell;

(c) detecting radiation emitted from a second emissive component of a biological sample disposed on a second surface of the flow cell using the detector and the corrective optics, wherein the first and second surfaces are in an arrangement whereby one of the surfaces is disposed between the detector and the other surface, wherein the corrective optics reduce aberration of detection at one of the surfaces due to the arrangement; and

(d) repeating steps (a)-(c) while maintaining the flow cell on the imaging station.

2. The method of claim 1, wherein the first surface is disposed between the detector and the second surface.

3. The method of claim 2, wherein a fluid is present between the two surfaces and the corrective optics reduce aberration due to the fluid.

4. The method of claim 3, wherein the detector is initially configured for diffraction-limited detection on the second surface and the corrective optics are configured for diffraction-limited detection on the first surface.

5. The method of claim 3, wherein the aberration comprises spherical aberration.

6. The method of claim 1, wherein the first surface is disposed between a source of radiation and the second surface.

7. The method of claim 1, wherein step (b) comprises inserting a corrective device between an objective and the flow cell, wherein the objective is disposed between the detector and the flow cell.

8. The method of claim 1, wherein step (b) comprises inserting a corrective lens between the detector and an objective, wherein the objective is disposed between the detector and the flow cell.

9. The method of claim 1, wherein step (a) and (c) comprise obtaining an image of the surfaces.

10. The method of claim 1, wherein steps (a) and (c) comprise focusing a line of radiation to an area on the first or second surface comprising the first or second emissive component.

11. The method of claim 1, wherein the emitted radiation is detected using epifluorescent excitation.

12. The method of claim 1, wherein an excitation radiation is directed towards the first and second surfaces by total internal reflection excitation.

13. The method of claim 12, wherein the excitation radiation and flow cell are configured to produce a total internal reflectance bounce that excites the first surface and the second surface.

14. The method of claim 1, wherein the first and second emissive components comprise fluorescently tagged nucleic acids of a nucleic acid sample.

15. The method of claim 1, wherein the first and second emissive components comprise separate features in arrays of features on the first and second surfaces, respectively.

16. The method of claim 15, wherein the separate features each comprise a population of identical molecules.

17. The method of claim 1, wherein a chromatic shift curve for the radiation detected in step (a) is the same as a chromatic shift curve for the radiation detected in step (b).

18. The method of claim 1, wherein a field curvature for detection in step (a) is the same as a field curvature for detection in step (b).

19. The method of claim 1, wherein a field distortion for detection in step (a) is the same as a field distortion for detection in step (b).

20. An imaging system for detecting radiation on a multi-surface flow cell, comprising:

a multi-surface flow cell having first and second emissive components of a biological sample disposed on respective first and second surfaces of the flow cell;

an optical train comprising an objective, imaging optics configured to focus the optical train on the first emissive component via the objective, and corrective optics configured to focus the optical train on the second emissive component and configured to reduce aberration of detection at the first or second emissive component;

a radiation source configured to direct excitation radiation towards the first and second emissive components;

detection optics configured to capture emitted radiation returned from the first and second emissive components via the optical train; and

a detector for detecting the captured radiation.

21. The imaging system of claim 20, wherein components of the corrective optics that are configured to focus the optical train on the second emissive component are separate from components of the corrective optics that are configured to reduce aberration of detection at the first or second emissive component.

22. The imaging system of claim 20, wherein the corrective optics comprise a corrective device configured to be inserted between the objective and the flow cell.

23. The imaging system of claim 20, wherein the corrective optics comprise a corrective lens configured to be inserted between the detector and the objective.

24. The imaging system of claim 20, wherein the radiation source is configured to direct the excitation radiation toward the first and second emissive components at several different wavelengths, and the detection optics and detector are configured to capture and detect the emitted radiation returned in response to each wavelength.

25. The imaging system of claim 20, wherein the flow cell includes a fluid in an interior volume and in contact with the biological samples.

26. The imaging system of claim 20, wherein the first and second emissive components comprise fluorescently tagged nucleic acids from a nucleic acid sample.

27. The imaging system of claim 20, wherein the first surface is disposed between the radiation source and the second surface.