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Autogenic living scaffolds and living tissue matrices: methods and uses thereof

  • US 8,043,614 B2
  • Filed: 03/09/2005
  • Issued: 10/25/2011
  • Est. Priority Date: 03/09/2004
  • Status: Active Grant
First Claim
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1. A three-dimensional cell-produced scaffold construct comprising extracellular matrix synthesized in vitro by fibroblast cells, wherein said construct is produced by a process comprising:

  • i) incubating the fibroblast cells at a concentration of at least about 200,000 cells/cm2 in defined chemical media containing components that promote proliferation and extracellular matrix synthesis of said fibroblast cells; and

    ii) isolating the construct from the media,wherein the cells are incubated for a period of time in the media that is sufficient to produce a construct having a thickness and porosity that enables cellular migration of additional cells throughout the construct wherein the construct has a failure strain of at least about 0.2.

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