Autogenic living scaffolds and living tissue matrices: methods and uses thereof
First Claim
Patent Images
1. A three-dimensional cell-produced scaffold construct comprising extracellular matrix synthesized in vitro by fibroblast cells, wherein said construct is produced by a process comprising:
- i) incubating the fibroblast cells at a concentration of at least about 200,000 cells/cm2 in defined chemical media containing components that promote proliferation and extracellular matrix synthesis of said fibroblast cells; and
ii) isolating the construct from the media,wherein the cells are incubated for a period of time in the media that is sufficient to produce a construct having a thickness and porosity that enables cellular migration of additional cells throughout the construct wherein the construct has a failure strain of at least about 0.2.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention is drawn to a 3-dimensional cell-produced scaffold construct comprising fibroblasts and the extracellular matrix that has been produced and arranged by these cells. The constructs promote the differentiation, dedifferentiation and/or transdifferentiation of cells and/or formation of tissue in vitro and in vivo, while at the same time promoting cell growth, proliferation, migration, and acquisition of in vivo-like morphology.
-
Citations
53 Claims
-
1. A three-dimensional cell-produced scaffold construct comprising extracellular matrix synthesized in vitro by fibroblast cells, wherein said construct is produced by a process comprising:
-
i) incubating the fibroblast cells at a concentration of at least about 200,000 cells/cm2 in defined chemical media containing components that promote proliferation and extracellular matrix synthesis of said fibroblast cells; and ii) isolating the construct from the media, wherein the cells are incubated for a period of time in the media that is sufficient to produce a construct having a thickness and porosity that enables cellular migration of additional cells throughout the construct wherein the construct has a failure strain of at least about 0.2. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 24, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 39, 40)
-
-
13. A three-dimensional cell-produced scaffold construct comprising fibroblast cells and extracellular matrix synthesized in vitro by said fibroblast cells, wherein said construct has a thickness and porosity that enables cellular migration throughout the construct, wherein the construct has a growth rate in the media such that it is capable of achieving a thickness of greater than 100 μ
- m within three weeks of growth, wherein the construct is made by a process comprising;
i) incubating the fibroblast cells at a concentration of at least about 200,000 cells/cm2 in defined chemical media containing components that promote proliferation and extracellular matrix synthesis of said fibroblast cells; and ii) isolating the construct from the media. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23, 25, 38, 41, 42, 43, 50)
- m within three weeks of growth, wherein the construct is made by a process comprising;
-
14. A three-dimensional cell-produced scaffold construct comprising extracellular matrix synthesized in vitro by fibroblast cells, wherein said construct is produced by a process comprising:
-
i) incubating the fibroblast cells at a concentration of at least about 200,000 cells/cm2 in defined chemical media containing components that promote proliferation and extracellular matrix synthesis of said fibroblast cells; and ii) isolating the construct from the media, wherein the construct has a porosity such that it is capable of achieving a thickness of greater than 200 μ
m.- View Dependent Claims (44, 45, 46, 47, 48, 49, 51, 52, 53)
-
Specification