Probe and primer for tubercle bacillus detection, and method of detecting human tubercle bacillus therewith
First Claim
1. An isolated or purified oligonucleotide consisting of a nucleic acid sequence selected from the nucleic acid sequence described in SEQ ID NO:
- 1, SEQ ID NO;
2, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
5, SEQ ID NO;
6, SEQ ID NO;
7, or SEQ ID NO;
8, or the complementary sequence thereof, wherein the oligonucleotide has a property of hybridizing with the nucleic acid sequence of IS6110 gene in Mycobacterium tuberculosis.
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Accused Products
Abstract
An object of the present invention is to provide a novel primer and prove for detecting tubercle bacillus capable of avoiding false positive, and an easy-to-use, rapid and high-sensitivity method for detecting human tubercle bacillus (Mycobacterium tuberculosis) using the same. The present invention relates to an oligonucleotide comprising a part of or an entire sequence of the nucleic acid sequence described in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8, or a part of or an entire sequence of the complementary sequence thereof, wherein the oligonucleotide has a property of hybridizing with the nucleic acid sequence of IS6110 gene in Mycobacterium tuberculosis, a primer and a probe containing the oligonucleotide for detecting Mycobacterium tuberculosis, and a method for detecting Mycobacterium tuberculosis using the primer and the probe.
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Citations
22 Claims
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1. An isolated or purified oligonucleotide consisting of a nucleic acid sequence selected from the nucleic acid sequence described in SEQ ID NO:
- 1, SEQ ID NO;
2, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
5, SEQ ID NO;
6, SEQ ID NO;
7, or SEQ ID NO;
8, or the complementary sequence thereof, wherein the oligonucleotide has a property of hybridizing with the nucleic acid sequence of IS6110 gene in Mycobacterium tuberculosis. - View Dependent Claims (14)
- 1, SEQ ID NO;
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2. A primer for detecting Mycobacterium tuberculosis comprising a nucleic acid sequence selected from the nucleic acid sequence described in SEQ ID NO:
- 1, SEQ ID NO;
2, SEQ ID NO;
3, SEQ ID NO;
4, or SEQ ID NO;
5, or the complementary sequence thereof, wherein the primer has a property of hybridizing with the nucleic acid sequence of IS6110 gene in Mycobacterium tuberculosis. - View Dependent Claims (3, 4, 15, 16)
- 1, SEQ ID NO;
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5. A probe for detecting Mycobacterium tuberculosis comprising a nucleic acid sequence selected from the nucleic acid sequence described in SEQ ID NO:
- 1, SEQ ID NO;
2, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
5, SEQ ID NO;
6, SEQ ID NO;
7, SEQ ID NO;
8, or SEQ ID NO;
11, or the complementary sequence thereof, wherein the probe has a property of hybridizing with the nucleic acid sequence of IS6110 gene in Mycobacterium tuberculosis. - View Dependent Claims (6, 7, 8, 13, 17, 18, 19, 20, 21)
- 1, SEQ ID NO;
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9. A kit for detecting Mycobacterium tuberculosis comprising:
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at least one of a primer pair or a probe; wherein the primer pair comprises a first primer comprising a nucleic acid sequence selected from the nucleic acid sequence described in SEQ ID NO;
1 or SEQ ID NO;
2, or the complementary sequence thereof, and a second primer comprising a nucleic sequence selected from the nucleic acid sequence described in SEQ ID NO;
3 or SEQ ID NO;
4, or the complementary sequence thereof;wherein the probe comprises a nucleic acid sequence selected from the nucleic acid sequence described in SEQ ID NO;
1, SEQ ID NO;
2, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
5, SEQ ID NO;
6, SEQ ID NO;
7, SEQ ID NO;
8, or SEQ ID NO;
11, or the complementary sequence thereof; andwherein the primer and the probe have a property of hybridizing with the nucleic acid sequence of IS6110 gene in Mycobacterium tuberculosis. - View Dependent Claims (10, 11, 12, 22)
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Specification